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Regulatory B Cells

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Francesca Mion

    1. Department of Medicine, University of Udine, Udine, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Silvia Tonon

    1. Department of Medicine, University of Udine, Udine, Italy

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2270)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Purification and Phenotypic Characterization of B Cell Subsets with Breg Traits

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Purification and Characterization of Murine MZ and T2-MZP Cells

      • M. Manuela Rosado, Alaitz Aranburu, Marco Scarsella, Simona Cascioli, Ezio Giorda, Rita Carsetti
      Pages 3-25

    3. Purification and Immune Phenotyping of B-1 Cells from Body Cavities of Mice

      • Vanessa Yenson, Nicole Baumgarth
      Pages 27-45

    4. Purification and Immunophenotypic Characterization of Murine Plasma Cells

      • Van Duc Dang, Simon Fillatreau, Andreia C. Lino
      Pages 47-59

    5. Purification of Murine and Human IL-10-Producing B Cells from Different Anatomical Compartments

      • Francesca Mion, Eleonora Martinis, Carlo E. M. Pucillo, Silvia Tonon
      Pages 61-76

    6. Purification and Immunophenotypic Characterization of Human CD19+CD24hiCD38hi and CD19+CD24hiCD27+ B Cells

      • Hannah F. Bradford, Claudia Mauri
      Pages 77-90

  3. Mechanisms of Immune Suppression by B Cells

    1. Front Matter

      Pages 91-91
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    2. Detection of IL-10 in Murine B Cells: In Vitro and In Vivo Techniques

      • Xiang Lin, Xiaohui Wang, Liwei Lu
      Pages 93-111

    3. Detection and Quantification of Transforming Growth Factor-β1 Produced by Murine B Cells: Pros and Cons of Different Techniques

      • Yoshiyuki Mishima, Akihiko Oka, Shunji Ishihara
      Pages 113-124

    4. IL-35 Detection in B Cells at the mRNA and Protein Level

      • Bhalchandra Mirlekar, Daniel Michaud, Yuliya Pylayeva-Gupta
      Pages 125-147

    5. Characterization and Activation of Fas Ligand-Producing Mouse B Cells and Their Killer Exosomes

      • Steven K. Lundy, Sophina H. Taitano, Luciën E. P. M. van der Vlugt
      Pages 149-178

    6. Characterization and Activity of TIM-1 and IL-10-Reporter Expressing Regulatory B Cells

      • Kanishka Mohib, David M. Rothstein, Qing Ding
      Pages 179-202

    7. New Method for the Expansion of Highly Purified Human Regulatory Granzyme B-Expressing B Cells

      • Mélanie Chesneau, Hoa Le Mai, Sophie Brouard
      Pages 203-216

    8. Characterization of the Cell Surface Phenotype and Regulatory Activity of B-Cell IgD Low (BDL) Regulatory B Cells

      • Mohamed Khalil, Avijit Ray, Bonnie N. Dittel
      Pages 217-231

  4. Methods for the Ex Vivo Expansion and Molecular Characterization of IL-10 Producing B Cells

    1. Front Matter

      Pages 233-233
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    2. Use of Toll-Like Receptor (TLR) Ligation to Characterize Human Regulatory B-Cells Subsets

      • Mathilde A. M. Chayé, Chiara Tontini, Arifa Ozir-Fazalalikhan, Astrid L. Voskamp, Hermelijn H. Smits
      Pages 235-261

    3. Use of Cocultures for the Study of Cellular Interactions Influencing B-Cell Regulatory Functions

      • Giada Pace, Viviana Valeri
      Pages 263-282

    4. Use of Inhibitory Compounds to Dissect the Molecular Pathways Involved in Regulatory B-Cell Differentiation

      • Maik Luu, Alexander Visekruna
      Pages 283-294

    5. Methods to Study the Transcriptome of Regulatory B Cells

      • Maud Maho-Vaillant, Sebastien Calbo
      Pages 295-305
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Keywords

About this book

 This second edition provides new and updated chapters useful for the study of Regulatory B cells. Organized in four sections, chapters detail basic methods for the isolation and immunophenotypical analysis of these cells, experimental approaches for the ex vivo generation/expansion of IL-10 producing B cells, molecular biology techniques for the analysis of IL-10 expression and production, and animal models mimicking pathologic settings. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Regulatory B Cells: Methods and Protocols, Second Edition aims to be useful to the scientific community and serve toclarify some unsolved aspects of Regulatory B Cells research.

Editors and Affiliations

  • Department of Medicine, University of Udine, Udine, Italy

    Francesca Mion, Silvia Tonon

Bibliographic Information

  • Book Title: Regulatory B Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Francesca Mion, Silvia Tonon

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1237-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1236-1Published: 22 January 2021

  • Softcover ISBN: 978-1-0716-1239-2Published: 22 January 2022

  • eBook ISBN: 978-1-0716-1237-8Published: 21 January 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 471

  • Number of Illustrations: 24 b/w illustrations, 66 illustrations in colour

  • Topics: Immunology

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