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Eosinophils

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Garry M. Walsh

    1. Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2241)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Molecular Biology of Eosinophils: Introduction

    • Paige Lacy, Helene F. Rosenberg, Garry M. Walsh
    Pages 1-14

  3. Functionally Active Eosinophil Purification from Peripheral Blood

    • Kiho Son, Paige Lacy, Manali Mukherjee
    Pages 15-25

  4. Eosinophil Purification from Peripheral Blood of Rhesus Monkeys

    • Fanny Legrand
    Pages 27-35

  5. Generation of Mouse Eosinophils in Tissue Culture from Unselected Bone Marrow Progenitors

    • Eric Mai, Ajinkya R. Limkar, Caroline M. Percopo, Helene F. Rosenberg
    Pages 37-47

  6. Detection of Mouse Eosinophils in Tissue by Flow Cytometry and Isolation by Fluorescence-Activated Cell Sorting (FACS)

    • Caroline M. Percopo, Ajinkya R. Limkar, Albert C. Sek, Helene F. Rosenberg
    Pages 49-58

  7. Mutant Mice and Animal Models of Airway Allergic Disease

    • Julyanne Brassard, David Marsolais, Marie-Renee Blanchet
    Pages 59-74

  8. Eosinophils in Asthma Models to House Dust Mite for Drug Development

    • François Daubeuf, Nelly Frossard
    Pages 75-87

  9. Analysis of Mouse Eosinophil Migration and Killing of Tumor Cells

    • Sharon Grisaru, Michal Itan, Ariel Munitz
    Pages 89-97

  10. Murine Models of Eosinophil Function in Fungal and Viral Infections

    • Meenakshi Tiwary, Kim S. LeMessurier, Amali E. Samarasinghe
    Pages 99-112

  11. Assays of Eosinophil Apoptosis and Phagocytic Uptake

    • Naomi N. Gachanja, David A. Dorward, Adriano G. Rossi, Christopher D. Lucas
    Pages 113-132Open Access

  12. In Vivo Eosinophil Expansion Using Gene Transfer by Electroporation

    • Emi Hamaguchi-Tsuru, Masataka Korenaga, Fabrizio Bruschi
    Pages 133-137

  13. Observation and Quantification of Eosinophil Motility

    • Karina T. Barretto, Deane F. Mosher, Nizar N. Jarjour, Colleen S. Curran, Stephane Esnault, Mats W. Johansson
    Pages 139-148

  14. Isolation and Functional Aspects of Eosinophil-Derived Exosomes

    • José Antonio Cañas, José Manuel Rodrigo-Muñoz, Victoria del Pozo
    Pages 149-159

  15. Interactions of Eosinophils with Nerves

    • Matthew G. Drake, Becky J. Proskocil, Zhenying Nie
    Pages 161-181

  16. Measurement of Eosinophil Kinetics In Vivo

    • Neda Farahi, Daniel Gillett, Chrystalla Loutsios, A. Michael Peters, Charlotte Summers, Edwin R. Chilvers
    Pages 183-191

  17. Detection of Eosinophil Extracellular DNA Traps

    • Marina Valente Barroso, Josiane Sabbadini Neves
    Pages 193-198

  18. Eosinophil Shape Change and Secretion

    • Sergei I. Ochkur, Elizabeth A. Jacobsen, Paige Lacy
    Pages 199-219

  19. Update on Eosinophil Interaction with Mast Cells: The Allergic Effector Unit

    • Roopesh Singh Gangwar, Hadas Pahima, Pier Giorgio Puzzovio, Francesca Levi-Schaffer
    Pages 221-242

  20. Assessing Phenotypic Heterogeneity in Intestinal Tissue Eosinophils

    • Courtney L. Olbrich, Leigha D. Larsen, Lisa A. Spencer
    Pages 243-255
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Keywords

About this book

This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte.

Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK

    Garry M. Walsh

Bibliographic Information

  • Book Title: Eosinophils

  • Book Subtitle: Methods and Protocols

  • Editors: Garry M. Walsh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1095-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1094-7Published: 24 January 2021

  • Softcover ISBN: 978-1-0716-1097-8Published: 24 January 2022

  • eBook ISBN: 978-1-0716-1095-4Published: 23 January 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 297

  • Number of Illustrations: 14 b/w illustrations, 51 illustrations in colour

  • Topics: Cell Biology, Immunology

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