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Germline Development in the Zebrafish

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2218)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
  2. Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish

    • Nana Ai, Lin Liu, Esther Shuk-Wa Lau, Anna Chung-Kwan Tse, Wei Ge
    Pages 1-9
  3. The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins

    • François Chauvigné, Alba Ferré, Joan Cerdà
    Pages 11-28
  4. Computer-Assisted Sperm Analysis to Test Environmental Toxicants

    • Tímea Kollár, Ákos Horváth, Zsolt Csenki-Bakos
    Pages 29-35
  5. Cryopreservation and Transplantation of Spermatogonial Stem Cells

    • Zoran Marinović, Jelena Lujić, Qian Li, Yoshiko Iwasaki, Béla Urbányi, Goro Yoshizaki et al.
    Pages 37-47
  6. Chemical Genetics: Manipulating the Germline with Small Molecules

    • Youngnam N. Jin, Randall T. Peterson
    Pages 61-73
  7. In Vitro Induction of Teleost PGCs

    • Vanesa Robles, David G. Valcarce, Marta F. Riesco
    Pages 75-83
  8. Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization

    • Jerónimo Miranda, Denhí Schnabel
    Pages 85-97
  9. Cryopreservation of Pooled Sperm Samples

    • Jennifer L. Matthews, Zoltan M. Varga
    Pages 99-115
  10. Quantifying Tissue Tension in the Granulosa Layer After Laser Surgery

    • Peng Xia, Carl-Philipp Heisenberg
    Pages 117-128
  11. In Vivo and Ex Vivo CT Imaging of Zebrafish Gonads

    • Christian Dullin, Louisa Habich
    Pages 129-135
  12. Detection of the Polar Body After Fertilization

    • Hua Ruan, Xiaogui Yi, Honghui Huang
    Pages 157-167
  13. Labeling the Micropylar Cell in Zebrafish Whole-Mount and Cryo-sectioned Follicles

    • Chaitanya Dingare, Petra A. Klemmt, Alina Niedzwetzki, Virginie Lecaudey
    Pages 169-183
  14. Visualization of Transcriptional Activity in Early Zebrafish Primordial Germ Cells

    • Fabio M. D’Orazio, Aleksandra Jasiulewicz, Yavor Hadzhiev, Ferenc Müller
    Pages 185-194

About this book

This volume details a wide range of methods, ranging from beginner through advanced, used to

further study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Institute for Developmental Biochemistry, University of Göttingen Medical Center Georg-August-Universität, Göttingen, Germany

    Roland Dosch

Bibliographic Information

  • Book Title: Germline Development in the Zebrafish

  • Book Subtitle: Methods and Protocols

  • Editors: Roland Dosch

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0970-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0969-9Published: 20 February 2021

  • Softcover ISBN: 978-1-0716-0972-9Published: 20 February 2022

  • eBook ISBN: 978-1-0716-0970-5Published: 19 February 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 383

  • Number of Illustrations: 11 b/w illustrations, 76 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access