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Vaccine Delivery Technology

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Blaine A. Pfeifer

    1. Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Andrew Hill

    1. Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2183)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Vaccine Delivery and Immune Response Basics

    • Andrew Hill, Marie Beitelshees, Blaine A. Pfeifer
    Pages 1-8

  3. Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) Using Bacterial Whole Cells as Molecular Probes

    • Sara Siris, Camilla A. Gladstone, Yanping Guo, Christopher L. Pinder, Robin J. Shattock, Paul F. McKay et al.
    Pages 9-18

  4. Use of Chlamydial Elementary Bodies as Probes to Isolate Pathogen-Specific Human Monoclonal Antibodies

    • Christopher L. Pinder, Paul F. McKay, Robin J. Shattock
    Pages 19-28

  5. Computational Antigen Discovery for Eukaryotic Pathogens Using Vacceed

    • Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis
    Pages 29-42

  6. Antigen Discovery in Bacterial Panproteomes

    • Daniel Yero, Oscar Conchillo-Solé, Xavier Daura
    Pages 43-62

  7. Alphavirus-Based Antigen Preparation

    • Kenneth Lundstrom
    Pages 63-81

  8. Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells

    • Mihaela-Olivia Dobrica, Catalin Lazar, Norica Branza-Nichita
    Pages 83-94Open Access

  9. Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System

    • Julie Harnischfeger, Lukas Käßer, Jan Zitzmann, Denise Salzig, Peter Czermak
    Pages 95-118

  10. High-Throughput Process Development for the Chromatographic Purification of Viral Antigens

    • Shaleem I. Jacob, Spyridon Konstantinidis, Daniel G. Bracewell
    Pages 119-182

  11. Production of Zika Virus Virus-Like Particles

    • Atichat Kuadkitkan, Suwipa Ramphan, Suchin Worawichawong, Wannapa Sornjai, Nitwara Wikan, Duncan R. Smith
    Pages 183-203

  12. Biosynthesis of Glycoconjugate Virus-like Particles (VLPs)

    • Kathryn K. Oi, Tom A. Kloter, Timothy G. Keys
    Pages 205-215

  13. Upstream and Downstream Processes for Viral Nanoplexes as Vaccines

    • Keven Lothert, Gregor Dekevic, Daniel Loewe, Denise Salzig, Peter Czermak, Michael W. Wolff
    Pages 217-248

  14. Whole-Cell Vaccine Preparation: Options and Perspectives

    • Punit Kumar, Sunita, Kashyap Kumar Dubey, Pratyoosh Shukla
    Pages 249-266

  15. O-Antigen Extraction, Purification, and Chemical Conjugation to a Carrier Protein

    • Francesca Micoli, Carlo Giannelli, Roberta Di Benedetto
    Pages 267-304

  16. Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines

    • Brittany R. Smith, Zhongwu Guo
    Pages 305-312

  17. Exploitation of Capsule Polymerases for Enzymatic Synthesis of Polysaccharide Antigens Used in Glycoconjugate Vaccines

    • Christa Litschko, Insa Budde, Monika Berger, Timm Fiebig
    Pages 313-330

  18. Attenuation Methods for Live Vaccines

    • Dipasree Hajra, Akshay Datey, Dipshikha Chakravortty
    Pages 331-356

  19. Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism

    • Yasmine Gabal, Joshua D. Ramsey
    Pages 357-366

  20. Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells

    • Friederike Eilts, Julie Harnischfeger, Daniel Loewe, Michael W. Wolff, Denise Salzig, Peter Czermak
    Pages 367-390
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Keywords

About this book

This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation of viral vaccines as VLPs; viral and non-viral gene delivery; needle-less or non-invasive delivery technology; vaccine storage; and vaccine administration and assessment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Vaccine Delivery Technology: Methods and Protocols is a valuable resource for both novice and expert researchers, in and outside the field, who would like to gain insight into the impactful fieldof vaccines. 

Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, USA

    Blaine A. Pfeifer, Andrew Hill

Bibliographic Information

  • Book Title: Vaccine Delivery Technology

  • Book Subtitle: Methods and Protocols

  • Editors: Blaine A. Pfeifer, Andrew Hill

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0795-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0794-7Published: 22 September 2020

  • Softcover ISBN: 978-1-0716-0797-8Published: 22 September 2020

  • eBook ISBN: 978-1-0716-0795-4Published: 21 September 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 590

  • Number of Illustrations: 22 b/w illustrations, 127 illustrations in colour

  • Topics: Chemistry/Food Science, general, Biotechnology, Biomaterials, Immunology, Biological Techniques, Pharmacology/Toxicology

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