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RNA Editing

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2181)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xv
  2. Substitutional RNA Editing in Plant Organelles

    • Mizuho Ichinose, Mamoru Sugita
    Pages 1-12
  3. Computational Detection of Plant RNA Editing Events

    • Alejandro A. Edera, M. Virginia Sanchez-Puerta
    Pages 13-34
  4. Discovering RNA Editing Events in Fungi

    • Huiquan Liu, Jin-Rong Xu
    Pages 35-50
  5. C-to-U RNA Editing: From Computational Detection to Experimental Validation

    • Taga Lerner, Mitchell Kluesner, Rafail Nikolaos Tasakis, Branden S. Moriarity, F. Nina Papavasiliou, Riccardo Pecori
    Pages 51-67
  6. Live-Cell Quantification of APOBEC1-Mediated RNA Editing: A Comparison of RNA Editing Assays

    • Martina Chieca, Serena Torrini, Silvestro G. Conticello
    Pages 69-81
  7. Adenosine-to-Inosine RNA Editing Enzyme ADAR and microRNAs

    • Kang Yuting, Dan Ding, Hisashi Iizasa
    Pages 83-95
  8. Quantitative Analysis of Adenosine-to-Inosine RNA Editing

    • Turnee N. Malik, Jean-Philippe Cartailler, Ronald B. Emeson
    Pages 97-111
  9. Discovering A-to-I RNA Editing Through Chemical Methodology “ICE-seq”

    • Masayuki Sakurai, Shunpei Okada, Hiroki Ueda, Yuxi Yang
    Pages 113-148
  10. ALU A-to-I RNA Editing: Millions of Sites and Many Open Questions

    • Amos A. Schaffer, Erez Y. Levanon
    Pages 149-162
  11. RNA Editing in Human and Mouse Tissues

    • Harini Srinivasan, Eng Piew Louis Kok, Meng How Tan
    Pages 163-176
  12. Bioinformatics Resources for RNA Editing

    • Maria Angela Diroma, Loredana Ciaccia, Graziano Pesole, Ernesto Picardi
    Pages 177-191
  13. High-Throughput Sequencing to Detect DNA-RNA Changes

    • Claudio Lo Giudice, Graziano Pesole, Ernesto Picardi
    Pages 193-212
  14. Detection of A-to-I Hyper-edited RNA Sequences

    • Roni Cohen-Fultheim, Erez Y. Levanon
    Pages 213-227
  15. Proteome Diversification by RNA Editing

    • Eli Eisenberg
    Pages 229-251
  16. MicroRNA Editing Detection and Function: A Combined In Silico and Experimental Approach for the Identification and Validation of Putative Oncogenic Targets

    • Valentina Tassinari, Valeriana Cesarini, Domenico Alessandro Silvestris, Andrea Scafidi, Lorenzo Cucina, Angela Gallo
    Pages 253-267
  17. RNA Editing in Interferonopathies

    • Loredana Frassinelli, Silvia Galardi, Silvia Anna Ciafrè, Alessandro Michienzi
    Pages 269-286
  18. The Role of RNA Editing in the Immune Response

    • Sadeem Ahmad, Xin Mu, Sun Hur
    Pages 287-307
  19. RNA Editing in Neurological and Neurodegenerative Disorders

    • Pedro Henrique Costa Cruz, Yukio Kawahara
    Pages 309-330
  20. New Frontiers for Site-Directed RNA Editing: Harnessing Endogenous ADARs

    • Tobias Merkle, Thorsten Stafforst
    Pages 331-349

About this book

This volume provides an overview about main RNA editing mechanisms, focusing on their functions in physiological as well as pathological conditions. Chapters guide readers through state- of-the art methodologies to investigate RNA editing through wet and dry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, RNA Editing: Methods and Protocols aims to ensure successful results in the further study of this vital field.

 


Editors and Affiliations

  • Department of Biosciences, Biotechnology and Biopharmaceutics, Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council, University of Bari “Aldo Moro”, Bari, Italy, Bari, Italy

    Ernesto Picardi, Graziano Pesole

Bibliographic Information

  • Book Title: RNA Editing

  • Book Subtitle: Methods and Protocols

  • Editors: Ernesto Picardi, Graziano Pesole

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0787-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0786-2Published: 30 July 2020

  • Softcover ISBN: 978-1-0716-0789-3Published: 31 July 2021

  • eBook ISBN: 978-1-0716-0787-9Published: 29 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 352

  • Number of Illustrations: 4 b/w illustrations, 43 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access