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Peptide and Protein Engineering

From Concepts to Biotechnological Applications

  • Book
  • © 2020

Overview

Editors:
  1. Olga Iranzo

    1. iSm2 UMR CNRS 7313, Aix-Marseille Université, Marseille, France

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  2. Ana Cecília Roque

    1. UCIBIO, Chemistry Department, School of Science and Technology, NOVA University of Lisbon, Caparica, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides important details essential for successful results
  • Contains key implementation advice from the experts

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. SEA-Mediated Ligation Is Accelerated at Mildly Acidic pH: Application to the Formation of Difficult Peptide Junctions

    • Marine Cargoët, Vincent Diemer, Laurent Raibaut, Elizabeth Lissy, Benoît Snella, Vangelis Agouridas et al.
    Pages 1-12

  3. The Problem of Aspartimide Formation During Protein Chemical Synthesis Using SEA-Mediated Ligation

    • Jennifer Bouchenna, Magalie Sénéchal, Hervé Drobecq, Jérôme Vicogne, Oleg Melnyk
    Pages 13-28

  4. Using the Interactive Tool of the Protein Chemical Synthesis Database

    • Vangelis Agouridas, Oleg Melnyk
    Pages 29-36

  5. Only a “Click” Away: Development of Arginine-Rich Peptide-Based Materials Using Click Chemistry

    • Mariana Barbosa, Fabíola Costa, Cátia Teixeira, M. Cristina L. Martins, Paula Gomes
    Pages 37-51

  6. Fluoroglycoproteins by Copper-Free Strain-Promoted Azide–Alkyne Cycloaddition

    • Pedro M. S. D. Cal, Gonçalo J. L. Bernardes, Omar Boutureira
    Pages 53-67

  7. Hybrid Silylated Peptides for the Design of Bio-functionalized Materials

    • Titouan Montheil, Cécile Echalier, Jean Martinez, Ahmad Mehdi, Gilles Subra
    Pages 69-92

  8. Synthesis of Peptide-Oligoethylene Glycol (OEG) Conjugates for Multivalent Modification of Nanomaterials

    • Daniel Pulido, Miriam Royo
    Pages 93-123

  9. Phage Display Methodologies

    • Agathe Urvoas, Philippe Minard, Patrice Soumillion
    Pages 125-151

  10. Whole-Bacterium Ribosome Display Selection for Isolation of Antibacterial Affitins

    • Ghislaine Béhar, Stanimir Kambarev, Jennifer Jazat, Barbara Mouratou, Frédéric Pecorari
    Pages 153-171

  11. CIS Display: DNA-Based Technology as a Platform for Discovery of Therapeutic Biologics

    • Ana Margarida Gonçalves Carvalho Dias
    Pages 173-188

  12. Building Scarless Gene Libraries in the Chromosome of Bacteria

    • Gol Mohammad Dorrazehi, Sebastian Worms, Jason Baby Chirakadavil, Johann Mignolet, Pascal Hols, Patrice Soumillion
    Pages 189-211

  13. Streptavidin (Sav)-Based Artificial Metalloenzymes: Cofactor Design Considerations and Large-Scale Expression of Host Protein Variants

    • Juliane Klehr, Jingming Zhao, Amanda Santos Kron, Thomas R. Ward, Valentin Köhler
    Pages 213-235

  14. Integrated Bacterial Production and Functional Screening of Expanded Cyclic Peptide Libraries for Identifying Chemical Rescuers of Pathogenic Protein Misfolding and Aggregation

    • Dafni C. Delivoria, Georgios Skretas
    Pages 237-266

  15. Site-Specific Incorporation of Non-canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli

    • Uchralbayar Tugel, Meritxell Galindo Casas, Birgit Wiltschi
    Pages 267-281

  16. Back Matter

    Pages 283-289
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Keywords

About this book

This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications. It opens with a section on chemical tools applied to the production or engineering of peptides and proteins, and concludes with a collection of chapters on biological approaches used to engineer structure and function in peptides and proteins. As a book in the Springer Protocols Handbooks series, chapters include the kind of detailed descriptions and tips necessary for successful results in practice. 

Authoritative and practical, Peptide and Protein Engineering: From Concepts to Biotechnological Applications will be of great use to scientists in academia and industry seeking a better understanding of the emerging principles and methodologies in peptide and protein engineering.

Editors and Affiliations

  • iSm2 UMR CNRS 7313, Aix-Marseille Université, Marseille, France

    Olga Iranzo

  • UCIBIO, Chemistry Department, School of Science and Technology, NOVA University of Lisbon, Caparica, Portugal

    Ana Cecília Roque

Bibliographic Information

  • Book Title: Peptide and Protein Engineering

  • Book Subtitle: From Concepts to Biotechnological Applications

  • Editors: Olga Iranzo, Ana Cecília Roque

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-0716-0720-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0719-0Published: 09 July 2020

  • Softcover ISBN: 978-1-0716-0722-0Published: 09 July 2021

  • eBook ISBN: 978-1-0716-0720-6Published: 09 July 2020

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XI, 289

  • Number of Illustrations: 17 b/w illustrations, 54 illustrations in colour

  • Topics: Protein Science, Industrial Chemistry/Chemical Engineering, Chemistry/Food Science, general, Biotechnology, Biomaterials

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