Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2153)
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Table of contents (36 protocols)
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Front Matter
About this book
This volume explores homologous recombination’s (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Homologous Recombination: Methods and Protocols is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair.
Editors and Affiliations
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Centro Andaluz de Biologı´a Molecular, y Medicina Regenerativa, CABIMER, Universidad de Sevilla, Sevilla, Spain
Andrés Aguilera
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Institut Curie, PSL Research University, CNRS, UMR3348, F-91405, Orsay, France
Aura Carreira
Bibliographic Information
Book Title: Homologous Recombination
Book Subtitle: Methods and Protocols
Editors: Andrés Aguilera, Aura Carreira
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0644-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-0643-8Published: 26 August 2020
Softcover ISBN: 978-1-0716-0646-9Published: 26 August 2021
eBook ISBN: 978-1-0716-0644-5Published: 25 August 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 557
Number of Illustrations: 35 b/w illustrations, 91 illustrations in colour
Topics: Human Genetics