Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2101)
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Table of contents (22 protocols)
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Front Matter
Keywords
About this book
This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers.
The chapter “Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Editors and Affiliations
Bibliographic Information
Book Title: Cytoskeleton Dynamics
Book Subtitle: Methods and Protocols
Editors: Helder Maiato
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0219-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0218-8Published: 27 December 2019
Softcover ISBN: 978-1-0716-0221-8Published: 27 December 2020
eBook ISBN: 978-1-0716-0219-5Published: 26 December 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 390
Number of Illustrations: 12 b/w illustrations, 72 illustrations in colour
Topics: Cell Biology, Protein Science