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Neural Reprogramming

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Henrik Ahlenius

    1. Stem Cells, Aging and Neurodegeneration Group, Faculty of Medicine, Department of Clinical Sciences, Neurology, Lund Stem Cell Center, Lund University, Lund, Sweden

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2352)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts

    • Juan M. Adrian-Segarra, Bettina Weigel, Moritz Mall
    Pages 1-12

  3. Direct In Vitro Reprogramming of Astrocytes into Induced Neurons

    • Nesrin Sharif, Filippo Calzolari, Benedikt Berninger
    Pages 13-29

  4. Direct Cell Reprogramming of Mouse Fibroblasts into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9

    • Boning Qiu, Ruben J. de Vries, Massimiliano Caiazzo
    Pages 31-43

  5. Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules

    • E Tian, Mingzi Zhang, Yanhong Shi
    Pages 45-55

  6. Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons

    • Amel Falco, Rocío Bartolomé-Cabrero, Sergio Gascón
    Pages 57-71

  7. Direct Conversion of Human Fibroblasts to Induced Neurons

    • Lucia Zhou-Yang, Sophie Eichhorner, Lukas Karbacher, Lena Böhnke, Larissa Traxler, Jerome Mertens
    Pages 73-96

  8. Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts

    • Emilie M. Legault, Janelle Drouin-Ouellet
    Pages 97-115

  9. Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs)

    • Ruiqi Hu, Xiaoting Zhu, Nan Yang
    Pages 117-126

  10. Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors

    • Keiko Imamura, Jitsutaro Kawaguchi, Tsugumine Shu, Haruhisa Inoue
    Pages 127-132

  11. Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons

    • Ella Quist, Henrik Ahlenius, Isaac Canals
    Pages 133-148

  12. Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression

    • Katrien Neyrinck, Juan A. García-León
    Pages 149-170

  13. Transcriptional Profiling During Neural Conversion

    • Yohannes Afeworki, Hannah Wollenzien, Michael S. Kareta
    Pages 171-181

  14. Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo

    • Srisaiyini Kidnapillai, Daniella Rylander Ottosson
    Pages 183-199

  15. Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods

    • Andreas Heuer
    Pages 201-226

  16. Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions

    • Juan M. Adrian-Segarra, Bettina Weigel, Moritz Mall
    Pages 227-236

  17. CRISPR/Cas9 Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders

    • Isaac Canals, Henrik Ahlenius
    Pages 237-251

  18. Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neuronal Cells

    • Giedre Kvist
    Pages 253-259

  19. Correction to: Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo

    • Srisaiyini Kidnapillai, Daniella Rylander Ottosson
    Pages C1-C1

  20. Back Matter

    Pages 261-263
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Keywords

About this book

This detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Neural Reprogramming: Methods and Protocols provides ample experimental experience and guidance for anyone, be it experienced researcher or beginner, to generate, validate, and apply reprogrammed neural cells in their research.

Editors and Affiliations

  • Stem Cells, Aging and Neurodegeneration Group, Faculty of Medicine, Department of Clinical Sciences, Neurology, Lund Stem Cell Center, Lund University, Lund, Sweden

    Henrik Ahlenius

Bibliographic Information

  • Book Title: Neural Reprogramming

  • Book Subtitle: Methods and Protocols

  • Editors: Henrik Ahlenius

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1601-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1600-0Published: 30 July 2021

  • Softcover ISBN: 978-1-0716-1603-1Published: 30 July 2022

  • eBook ISBN: 978-1-0716-1601-7Published: 29 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 263

  • Number of Illustrations: 3 b/w illustrations, 43 illustrations in colour

  • Topics: Neurosciences, Biomedical Engineering and Bioengineering

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