Overview
- Summarizes the various microfluidic-based single cell analysis methods
- Reviews the state-of-the-art techniques showcasing the pros and cons of each method in details
- Offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in reality
Part of the book series: Integrated Analytical Systems (ANASYS)
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Table of contents (9 chapters)
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Front Matter
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Back Matter
Keywords
About this book
This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Editors and Affiliations
About the editor
Bibliographic Information
Book Title: Microfluidics for Single-Cell Analysis
Editors: Jin-Ming Lin
Series Title: Integrated Analytical Systems
DOI: https://doi.org/10.1007/978-981-32-9729-6
Publisher: Springer Singapore
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer Nature Singapore Pte Ltd. 2019
Hardcover ISBN: 978-981-32-9728-9Published: 06 September 2019
Softcover ISBN: 978-981-32-9731-9Published: 06 September 2020
eBook ISBN: 978-981-32-9729-6Published: 28 August 2019
Series ISSN: 2196-4475
Series E-ISSN: 2196-4483
Edition Number: 1
Number of Pages: VII, 261
Number of Illustrations: 7 b/w illustrations, 99 illustrations in colour
Topics: Microengineering, Cell Cycle Analysis, Biomedical Engineering/Biotechnology, Analytical Chemistry