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Autophagy: Biology and Diseases

Technology and Methodology

  • Book
  • © 2021

Overview

Editors:
  1. Zhiping Xie

    1. Shanghai Jiao Tong University, Shanghai, China

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  • Systematically summarizing the cutting-edge knowledge in the study of autophagy
  • Introducing the methods and technologies in the study of autophagy
  • Serving as a critical reference tool for researchers and clinicians

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 1208)

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Table of contents (18 chapters)

  1. Front Matter

    Pages i-xiv
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  2. Investigating Autophagy: The Basics

    1. Front Matter

      Pages 1-1
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    2. The Classification and Basic Processes of Autophagy

      • Tiejian Nie, Lin Zhu, Qian Yang
      Pages 3-16

    3. Autophagosomal Membrane Origin and Formation

      • Yi Yang, Li Zheng, Xiaoxiang Zheng, Liang Ge
      Pages 17-42

    4. Phagophore Closure

      • Yongheng Liang
      Pages 43-53

    5. The Fusion Between Autophagic Vesicles and Lysosomes

      • Xiaoxia Liu, Qing Zhong
      Pages 55-66

    6. Autophagosome Trafficking

      • Jingjing Ye, Ming Zheng
      Pages 67-77

    7. Mechanisms of Selective Autophagy

      • Qi-Wen Fan, Xiang-Hua Yan
      Pages 79-98

    8. Similarities and Differences of Autophagy in Mammals, Plants, and Microbes

      • Fu-Cheng Lin, Huan-Bin Shi, Xiao-Hong Liu
      Pages 99-114

  3. Investigating Autophagy: Model Systems, Tools and Strategies

    1. Front Matter

      Pages 115-115
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    2. Monitoring Autophagy by Optical Microscopy

      • Yanrong Zheng, Xiangnan Zhang, Zhong Chen
      Pages 117-130

    3. Autophagic Flux Detection: Significance and Methods Involved

      • Xiao-Wei Zhang, Xiao-Xi Lv, Ji-Chao Zhou, Cai-Cai Jin, Lu-Yao Qiao, Zhuo-Wei Hu
      Pages 131-173

    4. Gene Manipulation Protocols in Autophagy

      • Rong Liu, Ren-Peng Guo, Yue-Guang Rong
      Pages 175-190

    5. MicroRNAs Regulating Autophagy in Neurodegeneration

      • Qingxuan Lai, Nikolai Kovzel, Ruslan Konovalov, Ilya A. Vinnikov
      Pages 191-264

    6. Biomarkers of Autophagy

      • Fang Lin, Yu-Ting Zhu, Zheng-Hong Qin
      Pages 265-287

    7. Chemical Autophagy Regulators

      • Ya-Ping Yang, Fen Wang, Chun-Feng Liu
      Pages 289-309

    8. Cell Models in Autophagy Research

      • Rui Huang, Shuyan Wu
      Pages 311-332

    9. Autophagy in Drosophila and Zebrafish

      • Xiuying Duan, Chao Tong
      Pages 333-356

    10. Screening for Genes Involved in Autophagy

      • Kefeng Lu, Huihui Li
      Pages 357-371

    11. Proteomics and Autophagy Research

      • Kefeng Lu, Huihui Li
      Pages 373-386
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Keywords

About this book

This book series consists of 3 volumes covering the basic science (Volume 1), clinical science (Volume 2) and the technology and methodology (Volume 3) of autophagy. Volume 3 focuses on the technical aspects of autophagy research. It is comprised of two parts. The first part discusses the basic process of autophagy, including its overall classification and individual stages in the life cycle of autophagosomes. The second part discusses the tools, strategies, and model systems in current autophagy research, including cell and animal models, detection and manipulation methods, as well as screening, genomic, proteomic and bioinformatic approaches. The book is written and edited by a team of active scientists. It is intended as a practical reference resource for interested researchers to get started on autophagy studies.

Editors and Affiliations

  • Shanghai Jiao Tong University, Shanghai, China

    Zhiping Xie

About the editor

Dr. Zhiping Xie is a professor in the School of Life Sciences and Biotechnology, Shanghai Jiao Tong University. He has been working in the autophagy field since the beginning of this century. His research focuses on the functions of Atg proteins, the formation process of autophagosomes, tools and methods in autophagy research, and intracellular protein trafficking. His work is published in journals including Autophagy, Molecular Biology of the Cell, BMC Biology, etc.



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