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Imaging Living Cells

  • Book
  • © 1999

Overview

Editors:
  1. Rosario Rizzuto

    1. Department of Experimental and Diagnostic Medicine, Section of General Pathology, Ferrara, Italy

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  2. Cristina Fasolato

    1. Department of Biomedical Sciences and CNR Center for the Study of Biomembranes, University of Padova, Padova, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • First manual describing novel labelling strategies * Easy to follow step-by-step instructions * Practical hints and experimental strategies are given for every protocol

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (16 chapters)

  1. Front Matter

    Pages I-XXI
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  2. The Instruments

    1. Front Matter

      Pages 1-1
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    2. Strategies for Quantitative Digital Imaging of Biological Activity in Living Cells with Ion-Sensitive Fluorescent Probes

      • William T. Mason, John Hoyland, Terry J. McCann, Baggi Somasundaram, William O’Brien
      Pages 3-29

    3. High Resolution 3-D Imaging of Living Cells by Image Restoration

      • Walter A. Carrington, Kevin E. Fogarty, Lawrence M. Lifshitz, Richard A. Tuft
      Pages 30-50

  3. Fluorescent Dyes

    1. Front Matter

      Pages 51-51
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    2. Imaging Calcium in the Cytoplasm and in Organelles with Fluorescent Dyes: General Principles

      • Aldebaran M. Hofer, Wim J. J. M. Scheenen
      Pages 53-91

    3. Confocal Calcium Imaging

      • Stephen Bolsover
      Pages 92-114

    4. Imaging Whole Organs — Single cell [Ca2+]i measurements in the perfused liver

      • Lawrence D. Robb-Gaspers, Paul A. Anderson, Andrew P. Thomas
      Pages 115-139

    5. Simultaneous Measurement of Membrane Current and Intracellular Calcium

      • Adam Zweifach, Markus Hoth, Richard S. Lewis
      Pages 140-163

    6. Quantitative Calcium Imaging in Brain Slices

      • Olga Garaschuk, Arthur Konnerth
      Pages 164-189

    7. Imaging of Cytosolic Sodium and pH Using SBFI and BCECF

      • Alec W. M. Simpson, Ram V. Sharma
      Pages 190-214

    8. Common Methods for Measuring Apoptotic Cell Death by Flow Cytometry

      • Ildo Nicoletti, Roberta Mannucci, Francesco Di Virgilio
      Pages 215-228

    9. Monitoring Synaptic Exocytosis and Endocytosis by Optical Techniques

      • Rosaria Ingrassia, Cristina Trabucchi, Andrea Bergamaschi, Antonio Malgaroli
      Pages 229-259

  4. Recombinant Probes

    1. Front Matter

      Pages 261-261
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    2. Targeting, Expressing and Calibrating Recombinant Aequorin

      • Marisa Brini, Paolo Pinton, Carlo Bastianutto
      Pages 263-283

    3. Imaging Ca2+ in Small Mammalian Cells with Recombinant Targeted Aequorin

      • Guy A. Rutter
      Pages 284-298

    4. Analysis of Regulated Gene Expression by Microinjection and Digital Luminescence Imaging of Single Living Cells

      • Guy A. Rutter, Michael R. H. White, Jeremy M. Tavaré
      Pages 299-326

    5. Imaging Green Fluorescent Proteins in Mammalian Cells

      • Marta Murgia, Rosario Rizzuto
      Pages 327-350

    6. The New Green Fluorescent Protein Mutants and their Applications

      • Francesca De Giorgi, Rosario Rizzuto
      Pages 351-361

    7. Imaging Green Fluorescent Protein in Transgenic Plants

      • Jim Haseloff
      Pages 362-394
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Keywords

About this book

Improved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together with classical probes. Novel labelling strategies, better software for image enhancement and analysis are now available and allow image acquisition with greater speed and precision. This lab manual, intended as for bench-top use, is suitable for both scientists and graduate students, combines an update on the most advanced imaging procedures with detailed protocols. Examples, cleverly selected from the wide repertoire of cell pyhsiology, cover different functional aspects such as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.

Editors and Affiliations

  • Department of Experimental and Diagnostic Medicine, Section of General Pathology, Ferrara, Italy

    Rosario Rizzuto

  • Department of Biomedical Sciences and CNR Center for the Study of Biomembranes, University of Padova, Padova, Italy

    Cristina Fasolato

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