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Animal Cell Culture: Principles and Practice

  • Book
  • © 2023

Overview

Authors:
  1. Shalini Mani

    1. Centre for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India

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  2. Manisha Singh

    1. Centre for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India

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  3. Anil Kumar

    1. Director, Education, Rani Lakshmi Bai Central Agricultural University, Jhansi, India

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  • Includes best practices and lab safety, ethical considerations, and troubleshooting for common issues
  • Perfect for junior researchers, research students, and established scientists seeking a go-to quick guide
  • One-stop reference or standard animal cell culture techniques

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Table of contents (21 chapters)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Overview to Animal Cell Culture

    • Shalini Mani
    Pages 1-9

  3. Cell Culture Laboratory

    • Anvi Jain, Aaru Gulati, Khushi R. Mittal, Shalini Mani
    Pages 11-52

  4. Good Laboratory Practices in Animal Cell Culture Laboratory and Biosafety Measures

    • Kumari Yukta, Mansi Agarwal, Mekhla Pandey, Khushi Mittal, Vidushi Srivastava, Shalini Mani
    Pages 53-64

  5. Managing Sterility in Animal Cell Culture Laboratory

    • Shalini Mani
    Pages 65-75

  6. Media and Buffer Preparation for Cell Culture

    • Sakshi Tyagi, Shalini Mani
    Pages 77-88

  7. Properties of Cultured Cells and Selection of Culture Media

    • Shalini Mani
    Pages 89-97

  8. Selection and Maintenance of Cultured Cells

    • Divya Jindal, Manisha Singh
    Pages 99-114

  9. Inoculation and Passaging of Adherent and Suspension Cells

    • Pranav Pancham, Divya Jindal, Manisha Singh
    Pages 115-130

  10. Counting of Cells

    • Divya Jindal, Manisha Singh
    Pages 131-145

  11. Cryopreservation of Cell Lines

    • Vinayak Agarwal, Manisha Singh
    Pages 147-161

  12. Resuscitation of Frozen Cell Lines

    • Vinayak Agarwal, Manisha Singh
    Pages 163-171

  13. Isolation and Culturing of Cells from Different Tissues

    • Sakshi Tyagi, Shalini Mani
    Pages 173-181

  14. Co-culture Techniques

    • Vinayak Agarwal, Manisha Singh, Vidushi Srivastava
    Pages 183-195

  15. 3D Cell Culture Techniques

    • Madhu Rani, Annu Devi, Shashi Prakash Singh, Rashmi Kumari, Anil Kumar
    Pages 197-212

  16. Stem Cell Culture Techniques

    • Rashmi Kumari, Madhu Rani, Amrita Nigam, Anil Kumar
    Pages 213-234

  17. Identification and Removal of Biological Contamination in the Media and Cell Suspensions

    • Vaishnavi Shishodia, Divya Jindal, Sarthak Sinha, Manisha Singh
    Pages 235-249

  18. Analysis of Cell Growth Kinetics in Suspension and Adherent Types of Cell Lines

    • Vaishnavi Shishodia, Divya Jindal, Sarthak Sinha, Manisha Singh
    Pages 251-265

  19. In Vitro Cytotoxicity Analysis: MTT/XTT, Trypan Blue Exclusion

    • Shalini Mani, Geeta Swargiary
    Pages 267-284

  20. Applications of Animal Cell Culture-Based Assays

    • Pallavi Shah, Anil Kumar, Rajkumar James Singh
    Pages 285-303
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Keywords

About this book

This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. 

Authors and Affiliations

  • Centre for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India

    Shalini Mani, Manisha Singh

  • Director, Education, Rani Lakshmi Bai Central Agricultural University, Jhansi, India

    Anil Kumar

About the authors

Dr. Shalini Mani is Associate Professor in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Manisha Singh is Assistant Professor and Lecturer in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Anil Kumar is Director of Education and Professor at RLB Central Agricultural University, Jhansi, India. 

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