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Myeloid Leukemia

Methods and Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Harry Iland

    1. Institute of Haematology, Royal Prince Alfred Hospital, Camperdown, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Mark Hertzberg

    1. Haematology Department, Westmead Hospital, Westmead, Australia

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    You can also search for this editor in PubMed   Google Scholar

  3. Paula Marlton

    1. Haematology Department, Princess Alexandra Hospital, Brisbane, Australia

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 125)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Isolation of RNA and DNA From Leukocytes and cDNA Synthesis

    • Joop H. Jansen, Bert A. van der Reijden
    Pages 1-11

  3. Cytogenetic and FISH Techniques in Myeloid Malignancies

    • Lynda J. Campbell
    Pages 13-26

  4. Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies

    • Christophe Picard, Monique Silvy, Jean Gabert
    Pages 27-68

  5. Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR

    • Susan Branford, Timothy Hughes
    Pages 69-92

  6. Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate

    • Susan Branford, Timothy Hughes
    Pages 93-106

  7. Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia

    • Lynda J. Campbell
    Pages 107-114

  8. Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR

    • Vincenzo Rossi, Laura Levati, Andrea Biondi
    Pages 115-126

  9. Diagnosis and Monitoring of PML-RARα-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR

    • Elisa Mokany, Alison V. Todd, Caroline J. Fuery, Tanya L. Applegate
    Pages 127-147

  10. Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR

    • Khalid Tobal, John A. Liu Yin
    Pages 149-161

  11. Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR

    • Bert A. van der Reijden, Joop H. Jansen
    Pages 163-175

  12. Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia

    • Jan Cools, Elizabeth H. Stover, D. Gary Gilliland
    Pages 177-187

  13. FLT3 Mutations in Acute Myeloid Leukemia

    • Hitoshi Kiyoi, Tomoki Naoe
    Pages 189-197

  14. WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes

    • Daniela Cilloni, Enrico Gottardi, Giuseppe Saglio
    Pages 199-211

  15. Classification of AML by DNA-Oligonucleotide Microarrays

    • Alexander Kohlmann, Wolfgang Kern, Wolfgang Hiddemann, Torsten Haferlach
    Pages 213-240

  16. Classification of AML Using a Monoclonal Antibody Microarray

    • Richard I. Christopherson, Kerryn Stoner, Nicole Barber, Larissa Belov, Adrian Woolfson, Mike Scott et al.
    Pages 241-251

  17. Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders

    • Peter J. Campbell, Linda M. Scott, Joanna E. Baxter, Anthony J. Bench, Anthony R. Green, Wendy N. Erber
    Pages 253-264

  18. Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia

    • Maurizio Martini, Luciana Teofili, Luigi M. Larocca
    Pages 265-273

  19. Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation

    • Thomas Lion, Franz Watzinger
    Pages 275-295

  20. Back Matter

    Pages 297-306
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Keywords

About this book

A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

Editors and Affiliations

  • Institute of Haematology, Royal Prince Alfred Hospital, Camperdown, Australia

    Harry Iland

  • Haematology Department, Westmead Hospital, Westmead, Australia

    Mark Hertzberg

  • Haematology Department, Princess Alexandra Hospital, Brisbane, Australia

    Paula Marlton

Bibliographic Information

  • Book Title: Myeloid Leukemia

  • Book Subtitle: Methods and Protocols

  • Editors: Harry Iland, Mark Hertzberg, Paula Marlton

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1597450170

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-485-2Published: 15 November 2005

  • Softcover ISBN: 978-1-60761-456-2Published: 21 November 2020

  • eBook ISBN: 978-1-59745-017-1Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XI, 307

  • Topics: Hematology

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