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Placenta and Trophoblast

Methods and Protocols, Volume I

  • Book
  • © 2006

Overview

Editors:
  1. Michael J. Soares

    1. Institute of Maternal-Fetal Biology Division of Cancer and Developmental Biology Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City

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    You can also search for this editor in PubMed   Google Scholar

  2. Joan S. Hunt

    1. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 121)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Overview I

      • Michael J. Soares, Joan S. Hunt
      Pages 3-6

  3. Methods for Studying Embryo Implantation and Uterine Biology

    1. Front Matter

      Pages 7-7
      Download chapter PDF

    2. Methodologies to Study Implantation in Mice

      • Kaushik Deb, Jeff Reese, Bibhash C. Paria
      Pages 9-34

    3. Blastocyst Culture

      • D. Randall Armant
      Pages 35-56

    4. Isolation of Hormone Responsive Uterine Stromal Cells

      • Virginia Rider
      Pages 57-67

    5. Rat Decidual Cell Cultures

      • Yan Gu, Geula Gibori
      Pages 69-78

    6. The Immortalization of Human Endometrial Cells

      • Graciela Krikun, Gil Mor, Charles Lockwood
      Pages 79-83

    7. Sheep Uterine Gland Knockout (UGKO) Model

      • Thomas E. Spencer, C. Allison Gray
      Pages 85-94

    8. A Baboon Model for Inducing Endometriosis

      • Asgerally T. Fazleabas
      Pages 95-99

    9. A Baboon Model for Simulating Pregnancy

      • Asgerally T. Fazleabas
      Pages 101-110

    10. The Common Marmoset Monkey as a Model for Implantation and Early Pregnancy Research

      • Almuth Einspanier, Kai Lieder, Ralf Einspanier, Bettina Husen
      Pages 111-121

  4. In Vitro Trophoblast and Placental Model Systems

    1. Front Matter

      Pages 123-123
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    2. Mouse Trophoblast Stem Cells

      • Jennifer Quinn, Tilo Kunath, Janet Rossant
      Pages 125-148

    3. Connexins and Trophoblast Cell Lineage Development

      • Mark Kibschull, Elke Winterhager
      Pages 149-158

    4. Rcho-1 Trophoblast Stem Cells

      • Namita Sahgal, Lindsey N. Canham, Brent Canham, Michael J. Soares
      Pages 159-178

    5. Bovine Trophoblast Cell Culture Systems

      • Kazuyoshi Hashizume, Arata Shimada, Haruo Nakano, Toru Takahashi
      Pages 179-188

    6. In Vitro Induction of Trophoblast from Human Embryonic Stem Cells

      • Ren-He Xu
      Pages 189-202

    7. Isolation and Culture of Term Human Trophoblast Cells

      • Margaret G. Petroff, Teresa A. Phillips, Hakhyun Ka, Judith L. Pace, Joan S. Hunt
      Pages 203-217
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Keywords

About this book

A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.

Reviews

"...provides step-by-step instructions for conducting research in mammalian embryology." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journal

 

"...an important contribution to the literature...recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journal

Editors and Affiliations

  • Institute of Maternal-Fetal Biology Division of Cancer and Developmental Biology Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City

    Michael J. Soares

  • Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City

    Joan S. Hunt

Bibliographic Information

  • Book Title: Placenta and Trophoblast

  • Book Subtitle: Methods and Protocols, Volume I

  • Editors: Michael J. Soares, Joan S. Hunt

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592599834

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-404-3Published: 01 November 2005

  • Softcover ISBN: 978-1-62703-813-3Published: 20 November 2014

  • eBook ISBN: 978-1-59259-983-7Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIX, 470

  • Topics: Obstetrics/Perinatology/Midwifery

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