Overview
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2846)
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About this book
This comprehensive guide delves into the protocols and strategies for investigating gene regulation both experimentally and computationally. It focuses on the use of Chromatin Immunoprecipitation (ChIP) coupled with next-generation sequencing, providing a robust framework for profiling DNA-binding proteins, examining DNA footprinting, and analyzing histone modifications. As part of the highly successful Methods in Molecular Biology series, this book offers introductions on the topics covered, detailed lists of the necessary materials and reagents, step-by-step laboratory protocols that are easy to follow, and expert tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Chromatin Immunoprecipitation: Methods and Protocols is the perfect resource for the new wave of genome biologists looking to master ChIP techniques.
Keywords
- Next-generation sequencing
- ChIP-seq
- DNA sequences
- Transcription factors
- Bioinformatics
- CUT&RUN
Editors and Affiliations
Bibliographic Information
Book Title: Chromatin Immunoprecipitation
Book Subtitle: Methods and Protocols
Editors: Franziska Greulich
Series Title: Methods in Molecular Biology
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024
Hardcover ISBN: 978-1-0716-4070-8Due: 13 September 2024
Softcover ISBN: 978-1-0716-4073-9Due: 13 September 2024
eBook ISBN: 978-1-0716-4071-5Due: 13 September 2024
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Illustrations: 11 b/w illustrations, 44 illustrations in colour