Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2840)
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Keywords
- Optobase
- Photoinducible proteins
- Photoactivatable yellow protein
- phytochromes
- optogenetic manipulation
About this book
This volume explores the latest advancements in the field of optogenetics and how it uses cellular light-sensing components and genetic engineering to control proteins and biological processes. The book chapters are organized into four parts. Part One focuses on intracellular optogenetic components for control of specific cell functions; Part Two looks at externally supplied light regulators that do not require genetic manipulation of target cells; Part Three highlights optogenetic control of organelles, and Part Four introduces technical tools required for light induction in optogenetic experiments, as well as a method for performing and analyzing optogenetic cell-cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Optogenetics: Methods and Protocols is a valuable resource to help researchers understand and apply the concepts of optogenetics and the underlying bioengineering principles, and establish the required technical light-illumination setups for administering light inputs and analysis of experimental outcomes.
Editors and Affiliations
Bibliographic Information
Book Title: Optogenetics
Book Subtitle: Methods and Protocols
Editors: Armin Baumschlager
Series Title: Methods in Molecular Biology
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024
Hardcover ISBN: 978-1-0716-4046-3Due: 02 September 2024
Softcover ISBN: 978-1-0716-4049-4Due: 02 September 2024
eBook ISBN: 978-1-0716-4047-0Due: 02 September 2024
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Illustrations: 6 b/w illustrations, 56 illustrations in colour