Overview
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2832)
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Keywords
- Stress-responsive genes
- Alternative splicing
- Plant biology
- Stress granules
- Reactive Oxygen Species
About this book
This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase–seq for identifying nucleosome occupancy, alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.
Editors and Affiliations
Bibliographic Information
Book Title: Plant Stress Tolerance
Book Subtitle: Methods and Protocols
Editors: Ramanjulu Sunkar
Series Title: Methods in Molecular Biology
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024
Hardcover ISBN: 978-1-0716-3972-6Due: 01 August 2024
Softcover ISBN: 978-1-0716-3975-7Due: 01 August 2024
eBook ISBN: 978-1-0716-3973-3Due: 01 August 2024
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: X, 343
Number of Illustrations: 6 b/w illustrations, 55 illustrations in colour