Overview
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2829)
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Keywords
- Baculovirus expression vector system (BEVS)
- Insect cell culture
- Baculoviral stocks
- Gene therapy vectors
- Recombinant viruses
- Vaccines
About this book
This detailed volume provides up-to-date guidance on techniques used to work with baculoviruses and insect cells. The book provides basic methods to create recombinant baculoviruses, to improve productivity, to produce a variety of products, to purify products, to quantify baculovirus stocks or to quantify product produced, and it concludes with alternative uses of either baculovirus or insect cells as tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Baculovirus: Methods and Protocols serves as an ideal guide for researchers looking to overcome some of the limitations associated with the early baculoviral vectors and cell lines.
Editors and Affiliations
Bibliographic Information
Book Title: Baculovirus
Book Subtitle: Methods and Protocols
Editors: Manon M.J. Cox
Series Title: Methods in Molecular Biology
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024
Hardcover ISBN: 978-1-0716-3960-3Due: 11 August 2024
Softcover ISBN: 978-1-0716-3963-4Due: 11 August 2024
eBook ISBN: 978-1-0716-3961-0Due: 11 August 2024
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Illustrations: 18 b/w illustrations, 46 illustrations in colour