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Mass Spectrometry Based Single Cell Proteomics

  • Book
  • Jul 2024

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2817)

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Keywords

  • protein extraction
  • high throughput
  • multiplexing
  • Data-dependent acquisition (DDA)
  • cell differentiation

About this book

This volume explores the latest advancements made in the field of mass spectrometry (MS) based single cell proteomics. The chapters in this book focus on technologies, methods, and biological applications and together cover the entire single cell proteomics analysis workflow, from sample collection and preparation through liquid chromatographic separation and mass spectrometric data acquisition to data analysis and interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Mass Spectrometry Based Single Cell Proteomics: Methods and Protocols is a valuable resource for all researchers interested in using these technologies and methods in their laboratories to continue making biological discoveries in this important field.

Editors and Affiliations

  • Proteomics Biomedicum, Karolinska Institute, Stockholm, Sweden

    Akos Vegvari

  • Faculty of Pharmacy, University of Helsinki, Helsinki, Finland

    Jaakko Teppo

  • Division of Physiological Chemistry, Karolinska Institute, Solna, Sweden

    Roman A. Zubarev

Bibliographic Information

  • Book Title: Mass Spectrometry Based Single Cell Proteomics

  • Editors: Akos Vegvari, Jaakko Teppo, Roman A. Zubarev

  • Series Title: Methods in Molecular Biology

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3933-7Due: 24 July 2024

  • Softcover ISBN: 978-1-0716-3936-8Due: 24 July 2024

  • eBook ISBN: 978-1-0716-3934-4Due: 24 July 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 225

  • Number of Illustrations: 2 b/w illustrations, 53 illustrations in colour

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