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Recombinant Protein Expression in Mammalian Cells

Methods and Protocols

  • Book
  • Jul 2024
  • Latest edition

Overview

Editors:
  1. David L. Hacker

    1. Ecole Polytechnique Fédérale de Lausan, Prangins, Switzerland

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2810)

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Keywords

  • Protein production
  • Transient recombinant protein production
  • Stable cell pool generation
  • SARS-CoV-2
  • Antibody expression
  • Clonal cell lines

About this book

This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifying host genes involved in protein production. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols, Second Edition serves as an ideal guide for researchers investigatingprotein structure and function and accelerating the discovery of new therapeutic proteins.

Editors and Affiliations

  • Ecole Polytechnique Fédérale de Lausan, Prangins, Switzerland

    David L. Hacker

Bibliographic Information

  • Book Title: Recombinant Protein Expression in Mammalian Cells

  • Book Subtitle: Methods and Protocols

  • Editors: David L. Hacker

  • Series Title: Methods in Molecular Biology

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3877-4Due: 20 July 2024

  • Softcover ISBN: 978-1-0716-3880-4Due: 20 July 2024

  • eBook ISBN: 978-1-0716-3878-1Due: 20 July 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 365

  • Number of Illustrations: 12 b/w illustrations, 57 illustrations in colour

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