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Patient-Derived Xenografts

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Mohamed I. Saad

    1. South Australian immunoGENomics Cancer Institute (SAiGENCI), University of Adelaide, Adelaide, Australia

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2806)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Patient-Derived Xenografts: Historical Evolution, Immunocompromised Host Models, and Translational Significance

    • James Thompson, Mohamed I. Saad
    Pages 1-8

  3. Patient-Derived Xenograft Models in Cancer Research: Methodology, Applications, and Future Prospects

    • Saeed Aslani, Mohamed I. Saad
    Pages 9-18

  4. Patient-Derived Xenografts: A Valuable Preclinical Model for Drug Development and Biomarker Discovery

    • Teresa Weng, Brendan J. Jenkins, Mohamed I. Saad
    Pages 19-30

  5. Patient-Derived Xenograft Models for Leukemias

    • Fiona C. Brown, Catherine L. Carmichael
    Pages 31-40

  6. Patient-Derived Orthotopic Xenograft Models for High-Grade Pediatric Brain Cancers

    • Jessica Buck, Hilary Hii, Raelene Endersby
    Pages 41-53

  7. Generation of Orthotopic and Subcutaneous Patient-Derived Xenograft Models from Diverse Clinical Tissue Samples of Pediatric Extracranial Solid Tumors

    • Kimberley M. Hanssen, Jamie I. Fletcher, Alvin Kamili
    Pages 55-74

  8. Development of Orthotopic Patient-Derived Xenograft Models of Pediatric Intracranial Tumors

    • Dannielle H. Upton, David S. Ziegler, Maria Tsoli
    Pages 75-90

  9. A Humanized Patient-Derived Xenograft Model for Pancreatic Cancer

    • Joanne Lundy
    Pages 91-100

  10. Development and Expansion of Patient-Derived Xenografts for Endometrial Cancer

    • Asmerom T. Sengal, Pamela M. Pollock
    Pages 101-115

  11. A Multiplexed Approach to Assess Small Cell Lung Cancer Subtype Heterogeneity in Primary and Patient-Derived Tumor Samples

    • Jin Ng, Emma Pan, Alex Johnston, Nina Tubau Ribera, Ariena Kersbergen, Jonas B. Hess et al.
    Pages 117-138

  12. Generation of Metastatic Cholangiocarcinoma Patient-Derived Xenograft Models

    • Mariana Yáñez-Bartolomé, Queralt Serra-Camprubí, Enrique J. Arenas, Marta Escorihuela, Florian Castet, Carles Fabregat-Franco et al.
    Pages 139-151

  13. Patient-Derived Xenograft Models for Translational Prostate Cancer Research and Drug Development

    • Lisa Kate Philp
    Pages 153-185

  14. Patient-Derived Xenograft Models for Ovarian Cancer

    • Trang Minh Tran, Gwo Yaw Ho, Simon Chu
    Pages 187-196

  15. Orthotopic MC-38 Allograft as a Robust Preclinical Model of Colorectal Carcinoma

    • Marina H. Yakou, Shoukat Afshar-Sterle, Matthias Ernst, Lisa A. Mielke
    Pages 197-207

  16. Assessing Protein Expression in Patient-Derived Xenografts Using Western Blotting

    • Bruna Rossi Herling, Moore Zhe Chen, Victoria M. McLeod
    Pages 209-218

  17. Analysis of Protein Interactions in Patient-Derived Xenografts Using Immunoprecipitation

    • Hozaifa Metwally, Maha M. Elbrashy
    Pages 219-227

  18. A Semiautomated Proteomics and Phosphoproteomics Protocol for the Identification of Novel Therapeutic Targets and Predictive Biomarkers in In Vivo Xenograft Models of Pediatric Cancers

    • Terry C. C. Lim Kam Sian, Christie Sun, Jason E. Cain, Joel R. Steele, Iresha Hanchapola, Stoyan Stoychev et al.
    Pages 229-242

  19. Back Matter

    Pages 243-244
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Keywords

About this book

This detailed volume explores the dynamic landscape of patient-derived xenograft (PDX) generation and applications. After a few chapters examining the evolution of PDXs, tracing their roots and contextualizing their significance in the broader narrative of cancer research, the book continues with methodologies for generating PDXs from diverse clinical tissue samples as well as for utilizing PDXs in various specific cancer types. From the development of humanized PDX models to the exploration of protein expression through advanced techniques including multiplex immunohistochemistry, Western blotting, and immunoprecipitation, the chapters offer a comprehensive toolkit for researchers and clinicians alike. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Patient-Derived Xenografts: Methods and Protocols serves as an ideal interdisciplinary guide for understanding the intricate processes that underscore translational cancer research.

Editors and Affiliations

  • South Australian immunoGENomics Cancer Institute (SAiGENCI), University of Adelaide, Adelaide, Australia

    Mohamed I. Saad

Bibliographic Information

  • Book Title: Patient-Derived Xenografts

  • Book Subtitle: Methods and Protocols

  • Editors: Mohamed I. Saad

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3858-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3857-6Published: 28 April 2024

  • Softcover ISBN: 978-1-0716-3860-6Due: 29 May 2024

  • eBook ISBN: 978-1-0716-3858-3Published: 27 April 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 244

  • Number of Illustrations: 5 b/w illustrations, 44 illustrations in colour

  • Topics: Cancer Research, Biomedicine, general

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