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Imaging Cell Signaling

  • Book
  • May 2024

Overview

Editors:
  1. Christoph Wuelfing

    1. Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Robert F. Murphy

    1. Computational Biology Department, Carnegie Mellon University, Pittsburgh, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2800)

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Keywords

  • iterative tissue staining
  • APEX-EM
  • correlative fluorescence
  • DNA origami
  • photoswitchable kinases

About this book

This volume explores recent innovations across the entire pipeline of imaging signal transduction, cell preparation, cellular manipulation, image acquisition, and computational analysis. The chapters in this book cover topics such as rapid preparation of living Drosophila pupal macrophages for ex vivo imaging; controlling the potency of T cell activation using an optically tuneable chimeric antigen receptor; in situ imaging of proteins using DNA-PAINT super-resolution microscopy; reconstructing signaling networks using biosensor barcoding; and morphological, spatial, and dynamic models for cellular components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Imaging Cell Signaling is a valuable resource that provides guidance to researchers looking to learn how to effectively tailor design projects to image, manipulate, and model signal transduction.



Editors and Affiliations

  • Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom

    Christoph Wuelfing

  • Computational Biology Department, Carnegie Mellon University, Pittsburgh, USA

    Robert F. Murphy

Bibliographic Information

  • Book Title: Imaging Cell Signaling

  • Editors: Christoph Wuelfing, Robert F. Murphy

  • Series Title: Methods in Molecular Biology

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3833-0Due: 07 June 2024

  • Softcover ISBN: 978-1-0716-3836-1Due: 07 June 2024

  • eBook ISBN: 978-1-0716-3834-7Due: 07 June 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 246

  • Number of Illustrations: 3 b/w illustrations, 73 illustrations in colour

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