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Characterization of Nanoparticles Intended for Drug Delivery

  • Book
  • © 2024
  • Latest edition

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Editors:
  1. Jeffrey D. Clogston

    1. Nanotechnology Characterization Laboratory Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, USA

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  2. Rachael M. Crist

    1. Nanotechnology Characterization Laboratory Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, USA

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  3. Marina A. Dobrovolskaia

    1. Nanotechnology Characterization Laboratory Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, USA

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  4. Stephan T. Stern

    1. Nanotechnology Characterization Laboratory Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2789)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xii
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Advancements in Nanoparticle Characterization

      • Rachael M. Crist, Jeffrey D. Clogston, Stephan T. Stern, Marina A. Dobrovolskaia
      Pages 3-17

  3. Physicochemical Characterization

    1. Front Matter

      Pages 19-19
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    2. Nanoparticle Size Distribution and Stability Assessment Using Asymmetric-Flow Field-Flow Fractionation

      • Matthew Hansen, Jeffrey D. Clogston
      Pages 21-29

    3. Assessment of Protein Binding Using Asymmetric-Flow Field-Flow Fractionation Combined with Multi-angle Light Scattering and Dynamic Light Scattering

      • Matthew Hansen, Jeffrey D. Clogston
      Pages 31-34

    4. Quantitation of Active Pharmaceutical Ingredients in Polymeric Drug Products Using Elemental Analysis

      • Jie Xu, Yingwen Hu, Jeffrey D. Clogston
      Pages 35-43

    5. Particle Size and Concentration Measurement Using the Spectradyne nCS1 Instrument

      • Alison Vermilya, Jeffrey D. Clogston
      Pages 45-52

    6. Measuring Size and Number Concentration of Metallic Nanoparticles Using spICP-MS

      • Matthew Hansen, Jeffrey D. Clogston
      Pages 53-66

    7. Ion Quantitation in Liposomal Products Using RP-HPLC with Charged Aerosol Detection

      • Jie Xu, Jeffrey D. Clogston
      Pages 67-73

    8. A Static Headspace Gas Chromatography Method for Quantitation of Residual Solvents in Nanoformulations

      • Krishna Kattel, Jeffrey D. Clogston
      Pages 75-83

  4. Immunotoxicity

    1. Front Matter

      Pages 85-85
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    2. Current Considerations and Practical Solutions for Overcoming Nanoparticle Interference with LAL Assays and Minimizing Endotoxin Contamination

      • Barry W. Neun, Marina A. Dobrovolskaia
      Pages 87-99

    3. Detection of Beta-Glucan Contamination in Nanoparticle Formulations

      • Barry W. Neun, Marina A. Dobrovolskaia
      Pages 101-108

    4. Detection of Intracellular Complement Activation by Nanoparticles in Human T Lymphocytes

      • Anna Ilinskaya, Ankit Shah, Andrew Van Dusen, Marina A. Dobrovolskaia
      Pages 109-120

    5. Analysis of Nanoparticles’ Potential to Induce Autoimmunity

      • Barry W. Neun, Timothy M. Potter, Christina Robinson, Simone Difilippantonio, Elijah Edmondson, Marina A. Dobrovolskaia
      Pages 121-127

    6. Analysis of Nanoparticles’ Effects on Drug-Induced Psoriasis

      • Ankit Shah, Chelsea Sanders, Simone Difilippantonio, Elijah Edmondson, Marina A. Dobrovolskaia
      Pages 129-135

    7. Detection of Nanoparticle-Mediated Total Oxidative Stress in T Cells Using CM-H2DCFDA Dye

      • Ankit Shah, Marina A. Dobrovolskaia
      Pages 137-143

    8. Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T Cells Using MitoSOX Red Dye

      • Ankit Shah, Marina A. Dobrovolskaia
      Pages 145-151

    9. Detection of Nanoparticle-Mediated Change in Mitochondrial Membrane Potential in T Cells Using JC-1 Dye

      • Ankit Shah, Marina A. Dobrovolskaia
      Pages 153-159
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Keywords

About this book

This third edition volume expands on the previous editions with new and updated discussions on the latest developments in endotoxin contamination, complex physicochemical properties, in vitro immunotoxicity traits, and in vitro drug release properties. Eight chapters in this book are dedicated to physicochemical characterization techniques and cover newer methods such as asymmetric-flow field-flow fractionation, single particle inductively coupled plasma mass spectrometry, and resistive pulse sensing. The next eighteen chapters explore the immunotoxicity of nanomaterials, including microbial contaminants such as endotoxin and beta-glucans, anti-PEG antibodies, autoimmunity, and immunosuppressive properties. The last two chapters talk about new pharmacology protocols, including a new technique to assess drug release and a tissue distribution assay using PEG immunohistochemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step instructions to reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, Characterization of Nanoparticles Intended for Drug Delivery, Third Edition is a valuable resource that will help both expert and novice researchers further enhance their understanding of this important and developing field.


Editors and Affiliations

  • Nanotechnology Characterization Laboratory Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, USA

    Jeffrey D. Clogston, Rachael M. Crist, Marina A. Dobrovolskaia, Stephan T. Stern

Bibliographic Information

  • Book Title: Characterization of Nanoparticles Intended for Drug Delivery

  • Editors: Jeffrey D. Clogston, Rachael M. Crist, Marina A. Dobrovolskaia, Stephan T. Stern

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3786-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Leidos Biomedical Research Inc. 2024

  • Hardcover ISBN: 978-1-0716-3785-2Published: 21 March 2024

  • Softcover ISBN: 978-1-0716-3788-3Due: 21 April 2024

  • eBook ISBN: 978-1-0716-3786-9Published: 20 March 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 324

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Pharmacology/Toxicology, Nanotechnology, Biomedicine, general

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