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New Technologies for Glutamate Interaction

Neurons and Glia

  • Book
  • © 2024

Overview

Editors:
  1. Maria Kukley

    1. Science Park of the UPV/EHU, Achucarro Basque Center for Neuroscience, Leioa, Spain

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 207)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. New Approaches to Study Glutamatergic Synapses Between Neurons

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Visualization of Glutamatergic Neurotransmission in Diverse Model Organisms with Genetically Encoded Indicators

      • Abhi Aggarwal, Joyce Chan, Amelia K. Waring, Adrian Negrean, Jonathan S. Marvin, Kaspar Podgorski et al.
      Pages 3-34

    3. Surface Glutamate Receptor Nanoscale Organization with Super-Resolution Microscopy (dSTORM)

      • Joana Ferreira, Laurent Groc
      Pages 35-52

    4. Ligand-Directed Chemical Labeling for Visualizing and Analyzing AMPA Receptors in Neurons

      • Shigeki Kiyonaka, Kyohei Soga, Kento Ojima, Hiroshi Nonaka, Itaru Hamachi
      Pages 53-70

    5. Live FRET-FLIM Imaging to Study Metabotropic Signaling via the NMDA Receptor

      • Mehreen Manikkoth, Kim Dore
      Pages 71-90

    6. Quantitative Analysis of Single Glutamatergic Vesicles in the Brain

      • Yuanmo Wang, Ajay Pradhan, Pankaj Gupta, Hanna Karlsson-Fernberg, Ann-Sofie Cans
      Pages 91-120

    7. Imaging Microtubule Network in Rodent Giant Glutamatergic Presynaptic Terminals

      • Laurent Guillaud
      Pages 121-133

    8. Photooxidation of Genetically Encoded MiniSOG-Fused VGLUT2 for Identification of Glutamatergic Synapses by Transmission and 3D Electron Microscopy

      • Andrew J. Flores, Anna Nilsson, Junru Hu, Mason R. Mackey, Mark H. Ellisman, Thomas S. Hnasko et al.
      Pages 135-151

  3. New Approaches to Study Role of Astrocytes at Neuronal Synapses

    1. Front Matter

      Pages 153-153
      Download chapter PDF

    2. Chemogenetic Approaches to Study Astrocytes at Glutamatergic Synapses

      • Liam Nestor, Yana Van Den Herrewegen, Zuner A. Bortolotto, Dimitri De Bundel, Ilse Smolders
      Pages 155-172

    3. Studying the Role of Astrocytes at Synapses Using Single-Cell Transcriptomics

      • Francisco Pestana, T. Grant Belgard, Thierry Voet, Matthew G. Holt
      Pages 173-206

    4. Analysis of Synaptic Glutamate Clearance as a Possible Indicator of Synaptic Health in the Degenerating Rodent Brain

      • Anton Dvorzhak, Rosemarie Grantyn
      Pages 207-227

    5. Computational Models of Astrocyte Function at Glutamatergic Synapses

      • Kerstin Lenk, Audrey Denizot, Barbara Genocchi, Ippa Seppälä, Marsa Taheri, Suhita Nadkarni
      Pages 229-263

  4. Technical Approaches to Study Fast Signaling at Neuron-Glia Synapses

    1. Front Matter

      Pages 265-265
      Download chapter PDF

    2. Electrophysiological Recordings of Oligodendroglia in Adult Mouse Brain Slices

      • Coram Guevara, Rodrigo Varas, María Cecilia Angulo, Fernando C. Ortiz
      Pages 267-282

    3. Synaptic Integration at Neuron-OPC Synapses

      • Wenjing Sun
      Pages 283-300

    4. Mapping Synaptic Inputs to Oligodendroglial Cells Using In Vivo Monosynaptic Viral Tracing

      • Belgin Yalçın, Michelle Monje
      Pages 301-312

    5. In Vivo Viral Gene Delivery to Manipulate Functional Properties of AMPA Receptors in Oligodendrocyte Lineage Cells

      • Ting-Jiun Chen, Bartosz Kula, Maria Kukley
      Pages 313-343

    6. Studying Synaptic Integration of Glioma Cells into Neural Circuits

      • Kiarash Shamardani, Kathryn R. Taylor, Tara Barron, Michelle Monje
      Pages 345-368
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Keywords

About this book

This volume explores the latest technologies used to study the co-existence of neuronal and neuron-glia synapses in the brain. The chapters in this volume are organized into three parts. Part One covers recent advancements in the technical approaches for studying glutamatergic signalling between neurons. Part Two highlights approaches to study the functional role of astrocytes at neuronal synapses. Part Three focuses on fast signalling at neuron-glia synapses, and highlights relevant methods for investigating these unusual synapses, including slice electrophysiology and in vivo gene delivery techniques. In the Neuromethods series style, all chapters contain key advices from experts in the field that are valuable for performing successful experiments on the topic in your laboratory.

Cutting-edge and thorough, New Technologies for Glutamate Interactions: Neurons and Glia is a valuable resource for both neuronal and glial physiologists who are interested in learning about and developing new methods to further understand neuronal and neuron-glia synaptic transmission.


Editors and Affiliations

  • Science Park of the UPV/EHU, Achucarro Basque Center for Neuroscience, Leioa, Spain

    Maria Kukley

Bibliographic Information

  • Book Title: New Technologies for Glutamate Interaction

  • Book Subtitle: Neurons and Glia

  • Editors: Maria Kukley

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-3742-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3741-8Published: 23 March 2024

  • Softcover ISBN: 978-1-0716-3744-9Due: 28 June 2024

  • eBook ISBN: 978-1-0716-3742-5Published: 21 March 2024

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIV, 371

  • Number of Illustrations: 6 b/w illustrations, 70 illustrations in colour

  • Topics: Neurosciences

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