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Plastids

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Eric Maréchal

    1. IRIG, CEA-Grenoble, UMR 5168 CNRS-CEA-INRAE-Univ. Grenoble A, Grenoble, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible result
  • Contain key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2776)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. Plastid Evolution, Plasticity, Structural, and Functional Diversity

    1. Front Matter

      Pages 1-1
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    2. How Did Thylakoids Emerge in Cyanobacteria, and How Were the Primary Chloroplast and Chromatophore Acquired?

      • Eric Maréchal
      Pages 3-20

    3. Complex Endosymbioses I: From Primary to Complex Plastids, Serial Endosymbiotic Events

      • Zoltán Füssy, Miroslav Oborník
      Pages 21-41

    4. Complex Endosymbiosis II: The Nonphotosynthetic Plastid of Apicomplexa Parasites (The Apicoplast) and Its Integrated Metabolism

      • Nyamekye Quansah, Sarah Charital, Yoshiki Yamaryo-Botté, Cyrille Y. Botté
      Pages 43-62

    5. Diversification of Plastid Structure and Function in Land Plants

      • Henrik Aronsson, Katalin Solymosi
      Pages 63-88

    6. The Main Functions of Plastids

      • Marcel Kuntz, Laura Dimnet, Sara Pullara, Lucas Moyet, Norbert Rolland
      Pages 89-106

    7. Plastid Transient and Stable Interactions with Other Cell Compartments

      • Stefanie J. Mueller-Schuessele, Sébastien Leterme, Morgane Michaud
      Pages 107-134

  3. Laboratory Protocols

    1. Front Matter

      Pages 135-135
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    2. Purification of Chloroplast Envelope, Thylakoids, and Stroma from Angiosperm Leaves

      • Maryse A. Block, Catherine Albrieux, Eric Maréchal
      Pages 137-149

    3. Isolation of the Inner and Outer Membranes of the Chloroplast Envelope from Angiosperms

      • Maryse A. Block, Eric Maréchal
      Pages 151-159

    4. Analysis of a Super-Complex at Contact Sites Between Mitochondria and Plastids

      • Morgane Michaud
      Pages 161-176

    5. Isolation of High-Quality Plastids from the Diatom Phaeodactylum tricornutum

      • Fan Hu, Wenxiu Yin, Teng Huang, Hanhua Hu
      Pages 177-183

    6. Determining the Subcellular Localization of Proteins in the Different Membranes of Diatom Secondary Plastid

      • Xiaojuan Liu, Yangmin Gong
      Pages 185-196

    7. Monitoring of Lipid Fluxes Between Host and Plastid-Bearing Apicomplexan Parasites

      • Sarah Charital, Amandine Lourdel, Nyamekye Quansah, Cyrille Y. Botté, Yoshiki Yamaryo-Botté
      Pages 197-204

    8. Extraction and Quantification of Lipids from Plant or Algae

      • Valérie Gros, Josselin Lupette, Juliette Jouhet
      Pages 205-230

    9. Quantitative Assessment of the Chloroplast Lipidome

      • Valérie Gros, Juliette Jouhet
      Pages 231-242

    10. The Use of Nanopore Sequencing to Analyze the Chloroplast Transcriptome Part I: Library Preparation

      • Sébastien Skiada, Alexandra Launay-Avon, Arnaud Liehrmann, Etienne Delannoy, Benoît Castandet
      Pages 243-257

    11. The Use of Nanopore Sequencing to Analyze the Chloroplast Transcriptome Part II: Bioinformatic Analyzes and Virtual RNA Blots

      • Etienne Delannoy, Arnaud Liehrmann, Benoît Castandet
      Pages 259-267

    12. Targeted Gene Editing of Nuclear-Encoded Plastid Proteins in Phaeodactylum tricornutum via CRISPR/Cas9

      • Cécile Giustini, Jhoanell Angulo, Florence Courtois, Guillaume Allorent
      Pages 269-287
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Keywords

About this book

This second edition volume expands on the previous edition with new and updated chapters that explore our current view on plastid evolution, structure, and function. The chapters in this book are organized into three parts and cover topics such as plastid evolution, plasticity, and functional and structural diversity; techniques used to visualize, fractionate, purify, and study primary plastids from plant materials, and secondary plastids; and methods to analyze plastids by integrated biology strategies based on genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Plastids: Methods and Protocols, Second Edition is a valuable resource that willhelp students, engineers, and researchers further explore and understand this fascinating organelle.

 

Editors and Affiliations

  • IRIG, CEA-Grenoble, UMR 5168 CNRS-CEA-INRAE-Univ. Grenoble A, Grenoble, France

    Eric Maréchal

Bibliographic Information

  • Book Title: Plastids

  • Book Subtitle: Methods and Protocols

  • Editors: Eric Maréchal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3726-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3725-8Due: 28 May 2024

  • Softcover ISBN: 978-1-0716-3728-9Due: 28 May 2024

  • eBook ISBN: 978-1-0716-3726-5Published: 19 March 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 322

  • Number of Illustrations: 12 b/w illustrations, 45 illustrations in colour

  • Topics: Plant Sciences

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