Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2652)
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Table of contents (24 protocols)
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Front Matter
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Vector Design and Protein Expression
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Front Matter
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Recombinant Protein Purification and Stabilization
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Front Matter
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Protein Bio-Interaction Analysis
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Front Matter
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Keywords
About this book
This volume explores the latest integrated bioprocesses and technologies used to study the production of the target recombinant protein of therapeutic or diagnostic interest; its isolation, purification, and stabilization; and the bio-interaction and structural analyses. The chapters in this book are organized into four parts. Part One covers production methods of soluble and membrane proteins in prokaryotic and eukaryotic expression systems, such as Lactococcus lactis and Escherichia coli. Part Two describes traditional and novel approaches for recombinant protein purification and stabilization, and buffers and additives. Part Three discusses automated methods in structural biology based on in silico approaches; and Part Four provides examples of advanced protein investigation methodologies to assess structural analysis such as high-throughput protein crystallization and time-resolved serial crystallography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Advanced Methods in Structural Biology is a valuable resource to those in academia (i.e., graduate students and postdoctoral researchers) and researchers in the pharmaceutical industry who wish to learn more about this developing field.
Chapter 5 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Editors and Affiliations
Bibliographic Information
Book Title: Advanced Methods in Structural Biology
Editors: Ângela Sousa, Luis Passarinha
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-3147-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023
Hardcover ISBN: 978-1-0716-3146-1Published: 25 April 2023
Softcover ISBN: 978-1-0716-3149-2Published: 26 April 2024
eBook ISBN: 978-1-0716-3147-8Published: 24 April 2023
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 467
Number of Illustrations: 21 b/w illustrations, 82 illustrations in colour
Topics: Life Sciences, general