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Pyroptosis

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Susan L. Fink

    1. Department of Laboratory Medicine and Pathology, University of Washington, Seattle, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2641)

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About this book

This volume explores techniques used to study and understand the latest research on pyroptosis. The chapters in this book cover topics such as experimental methods to induce pyroptosis via inflammasomes; approaches to identifying inflammasome activation in humans; ways to induce and detect pore formation; and techniques to evaluate cellular outcomes of pyroptotic cell death. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Pyroptosis: Methods and Protocols is a valuable tool for researchers who want to expand their knowledge of this developing field. It will be especially beneficial for those working in the fields of immunology, cancer biology, microbiology, and cell biology.


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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Molecular Mechanisms of Pyroptosis

    • Marisa J. Anderson, Andreas B. den Hartigh, Susan L. Fink
    Pages 1-16

  3. Assessment of NLRP3 Inflammasome Activation and NLRP3-NEK7 Complex Assembly

    • Nathan Kelley, Yuan He
    Pages 17-26

  4. Inducing Pyroptosis with FlaTox, RodTox, or NeedleTox

    • William P. Scott, Isabella Rauch
    Pages 27-36

  5. Inducing Pyroptosis Via the Pyrin Inflammasome

    • Pauline Bronnec, Jeremy Sousa, Thomas Henry
    Pages 37-47

  6. Caspase-4 Activation and Recruitment to Intracellular Gram-Negative Bacteria

    • Marisa Dilucca, Petr Broz
    Pages 49-65

  7. CARD8 Inflammasome Activation by HIV-1 Protease

    • Kolin M. Clark, Qiankun Wang, Liang Shan
    Pages 67-79

  8. Pyroptosis Markers in Human Primary Specimens: Quantification of Intracellular ASC Specks by Imaging Flow Cytometry and Extracellular Oxidized Mitochondrial by ELISA

    • Amy F. McLemore, Silvia L. Lage, Venina M. Dominical, Matthew A. Rodrigues, Kathy L. McGraw
    Pages 81-100

  9. Identifying Inflammasome Activation in Human Tissues

    • Eleonora Mezzaroma, Adolfo Mauro, Stefano Toldo
    Pages 101-113

  10. Analyzing Caspase-8-Dependent GSDMD Cleavage in Response to Yersinia Infection

    • Felicia Hui Min Chan, Kaiwen W. Chen
    Pages 115-124

  11. Biochemical Methods for Assessing Gasdermin D Inactivation in Macrophages

    • Fiachra Humphries
    Pages 125-133

  12. Detection of Gasdermin C-Mediated Cancer Cell Pyroptosis

    • Junwei Hou, Shuanglian Wang, Rui Miao, Xin Zhang, Mien-Chie Hung
    Pages 135-146

  13. Inducing Pyroptosis via Gasdermin B and Gasdermin E Cleavage

    • Zhiwei Zhou, Yupeng Wang, Feng Shao
    Pages 147-161

  14. Assessing Interleukin-1β Activation During Pyroptosis

    • Doris L. LaRock, Christopher N. LaRock
    Pages 163-169

  15. Monitoring Calcium Fluxes and Lysosome Exocytosis During Pyroptosis

    • Wendy P. Loomis, Tessa Bergsbaken
    Pages 171-178

  16. Assessing Cytoskeletal Destruction During Pyroptosis

    • Michael A. Davis, Michael Gale Jr
    Pages 179-188

  17. Assessment of HMGB1 Release During Experimentally Induced Pyroptosis

    • A. E. Foley, S. Sulstarova, N. M. Goldenberg
    Pages 189-202

  18. Time-Lapse Imaging of Inflammasome-Dependent Cell Death and Extrusion in Enteroid-Derived Intestinal Epithelial Monolayers

    • Petra Geiser, Jorik M. van Rijn, Mikael E. Sellin
    Pages 203-221

  19. Back Matter

    Pages 223-224
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Editors and Affiliations

  • Department of Laboratory Medicine and Pathology, University of Washington, Seattle, USA

    Susan L. Fink

Bibliographic Information

  • Book Title: Pyroptosis

  • Book Subtitle: Methods and Protocols

  • Editors: Susan L. Fink

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3040-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3039-6Published: 20 April 2023

  • Softcover ISBN: 978-1-0716-3042-6Published: 21 April 2024

  • eBook ISBN: 978-1-0716-3040-2Published: 19 April 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 224

  • Number of Illustrations: 6 b/w illustrations, 30 illustrations in colour

  • Topics: Cell Biology, Cell Biology

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