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Spectral and Imaging Cytometry

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Natasha S. Barteneva

    1. Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan

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    You can also search for this editor in PubMed   Google Scholar

  2. Ivan A. Vorobjev

    1. Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2635)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Spectral Flow Cytometry

    1. Front Matter

      Pages 1-1
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    2. Development of Spectral Imaging Cytometry

      • Ivan A. Vorobjev, Aigul Kussanova, Natasha S. Barteneva
      Pages 3-22Open Access

    3. Using Virtual Filtering Approach to Discriminate Microalgae by Spectral Flow Cytometer

      • Natasha S. Barteneva, Aigul Kussanova, Veronika Dashkova, Ayagoz Meirkhanova, Ivan A. Vorobjev
      Pages 23-40Open Access

  3. Imaging Flow Cytometry: Imagestream Systems

    1. Front Matter

      Pages 41-41
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    2. Imaging Flow Cytometric Analysis of Primary Bone Marrow Erythroblastic Islands

      • Joshua Tay, Kavita Bisht, Ingrid G. Winkler, Jean-Pierre Levesque
      Pages 43-61

    3. Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant Dictyostelium

      • Amanda Welin, Dario Hüsler, Hubert Hilbi
      Pages 63-85

    4. Imaging Flow Cytometry of Multi-Nuclearity

      • Ivan A. Vorobjev, Sultan Bekbayev, Adil Temirgaliyev, Madina Tlegenova, Natasha S. Barteneva
      Pages 87-101Open Access

    5. The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay

      • Ruth C. Wilkins, Matthew Rodrigues, Lindsay A. Beaton-Green
      Pages 103-122

    6. High-Throughput γ-H2AX Assay Using Imaging Flow Cytometry

      • Younghyun Lee, Qi Wang, Ki Moon Seong, Helen C. Turner
      Pages 123-134

    7. Label-Free Identification of Persistent Particles in Association with Primary Immune Cells by Imaging Flow Cytometry

      • Bradley Vis, Jonathan J. Powell, Rachel E. Hewitt
      Pages 135-148

    8. “Immuno-FlowFISH”: Applications for Chronic Lymphocytic Leukemia

      • Henry Y. L. Hui, Wendy N. Erber, Kathy A. Fuller
      Pages 149-171

    9. Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry

      • Inbal Wortzel, Ziv Porat
      Pages 173-184

    10. Flow Imaging of the Inflammasome: Evaluating ASC Speck Characteristics and Caspase-1 Activity

      • Abhinit Nagar, Jonathan A. Harton
      Pages 185-202

    11. Quantitative Analysis of Latex Beads Phagocytosis by Human Macrophages Using Imaging Flow Cytometry with Extended Depth of Field (EDF)

      • Ekaterina Pavlova, Daria Shaposhnikova, Svetlana Petrichuk, Tatiana Radygina, Maria Erokhina
      Pages 203-215

  4. Imaging Flow Cytometry: FlowCam

    1. Front Matter

      Pages 217-217
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    2. FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry

      • Kathryn H. Roache-Johnson, Nicole R. Stephens
      Pages 219-244

    3. Optimizing FlowCam Imaging Flow Cytometry Operation for Classification and Quantification of Microcystis Morphospecies

      • Dmitry Malashenkov, Veronika Dashkova, Ivan A. Vorobjev, Natasha S. Barteneva
      Pages 245-258

  5. Back Matter

    Pages 259-260
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Keywords

About this book

This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations.


Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. 

Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.




Editors and Affiliations

  • Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan

    Natasha S. Barteneva, Ivan A. Vorobjev

Bibliographic Information

  • Book Title: Spectral and Imaging Cytometry

  • Book Subtitle: Methods and Protocols

  • Editors: Natasha S. Barteneva, Ivan A. Vorobjev

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3020-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3019-8Published: 20 April 2023

  • Softcover ISBN: 978-1-0716-3022-8Published: 21 April 2024

  • eBook ISBN: 978-1-0716-3020-4Published: 19 April 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 260

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Cell Biology

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