Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2623)
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Table of contents (16 protocols)
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Front Matter
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Methods for the Cell Biological Assessment of Dynein Function: Fungal Systems
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Front Matter
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Methods for the Cell Biological Assessment of Dynein Function: Metazoan Systems
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Front Matter
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At the Crossroads: Combined In-Cell and In Vitro Methods to Measure Dynein Function
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Front Matter
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Methods for the Biochemical Reconstitution of Active Dynein Assemblies
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Front Matter
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Methods to Measure Motility and Force Generation of Dynein Assemblies
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Front Matter
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About this book
This volume provides a collection of protocols from leading researchers in the dynein field. The enclosed chapters guide readers through a broad range of powerful techniques that can be used to understand the complexities of dynein activity and regulation. These include how to perform live-cell microscopy on various fungi and metazoan cells, how to purify, assemble, and measure the force generation and motility characteristics of various dynein assemblies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Dynein: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
Editors and Affiliations
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Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, USA
Steven M. Markus
Bibliographic Information
Book Title: Dynein
Book Subtitle: Methods and Protocols
Editors: Steven M. Markus
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2958-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023
Hardcover ISBN: 978-1-0716-2957-4Published: 06 January 2023
Softcover ISBN: 978-1-0716-2960-4Published: 07 January 2024
eBook ISBN: 978-1-0716-2958-1Published: 05 January 2023
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 282
Number of Illustrations: 9 b/w illustrations, 55 illustrations in colour
Topics: Cell Biology