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Dynein

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Steven M. Markus

    1. Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2623)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Methods for the Cell Biological Assessment of Dynein Function: Fungal Systems

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Live-Cell Imaging of Dynein-Mediated Cargo Transport in Aspergillus nidulans

      • Rongde Qiu, Jun Zhang, Dennis McDaniel, Miguel A. Peñalva, Xin Xiang
      Pages 3-23

    3. Using Budding Yeast as a Model to Understand Dynein-Mediated Cargo Transport

      • Kari H. Ecklund, Matthew G. Marzo, Steven M. Markus
      Pages 25-42

  3. Methods for the Cell Biological Assessment of Dynein Function: Metazoan Systems

    1. Front Matter

      Pages 43-43
      Download chapter PDF

    2. Employing Live-Cell Imaging to Study Motor-Mediated Transport

      • Sydney E. Cason, Adam R. Fenton, Erika L. F. Holzbaur
      Pages 45-59

    3. Assessment of Dynein-Mediated Nuclear Migration in the Developing Cortex by Live-Tissue Microscopy

      • Haw-Yuan Cheng, Fang-Shin Nian, Yi-Wen Ou, Jin-Wu Tsai
      Pages 61-71

    4. Using Optogenetics to Spatially Control Cortical Dynein Activity in Mitotic Human Cells

      • Tomomi Kiyomitsu
      Pages 73-85

    5. Visualization of Single Dynein Molecules in Mammalian Cells

      • Nireekshit Addanki Tirumala, Vaishnavi Ananthanarayanan
      Pages 87-93

  4. At the Crossroads: Combined In-Cell and In Vitro Methods to Measure Dynein Function

    1. Front Matter

      Pages 95-95
      Download chapter PDF

    2. Single-Molecule Studies of Motor Adaptors Using Cell Lysates

      • Adam R. Fenton, Sydney E. Cason, Erika L. F. Holzbaur
      Pages 97-111

    3. Reconstitution of Organelle Transport Along Microtubules In Vitro

      • Daniel Beaudet, Adam G. Hendricks
      Pages 113-132

  5. Methods for the Biochemical Reconstitution of Active Dynein Assemblies

    1. Front Matter

      Pages 133-133
      Download chapter PDF

    2. Reconstitution of Dynein/Dynactin Transport Using Recombinant Dynein

      • Clinton K. Lau
      Pages 135-156

    3. Fast and Easy Transient Mammalian Cell Expression and Purification of Cytoplasmic Dynein

      • Akane Furuta, Ken’ya Furuta
      Pages 157-173

  6. Methods to Measure Motility and Force Generation of Dynein Assemblies

    1. Front Matter

      Pages 175-175
      Download chapter PDF

    2. High-Resolution Tracking of Dynein-Dynactin-BicD2 Complexes

      • Qingzhou Feng, Allison M. Gicking, William O. Hancock
      Pages 177-186

    3. In Vivo Trapping of Latex Bead Phagosomes for Quantitative Force Measurements

      • Paulomi Sanghavi, Arpan Rai, Roop Mallik
      Pages 187-200

    4. Studying Dynein Mechanochemistry with an Optical Trap

      • Emre Kusakci, Ahmet Yildiz
      Pages 201-219

    5. Measurements of the Force-Dependent Detachment Rates of Cytoplasmic Dynein from Microtubules

      • Xinglei Liu, Lu Rao, Arne Gennerich
      Pages 221-238
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Keywords

About this book

This volume provides a collection of protocols from leading researchers in the dynein field. The enclosed chapters guide readers through a broad range of powerful techniques that can be used to understand the complexities of dynein activity and regulation. These include how to perform live-cell microscopy on various fungi and metazoan cells, how to purify, assemble, and measure the force generation and motility characteristics of various dynein assemblies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Cutting-edge and thorough, Dynein: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.

Editors and Affiliations

  • Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, USA

    Steven M. Markus

Bibliographic Information

  • Book Title: Dynein

  • Book Subtitle: Methods and Protocols

  • Editors: Steven M. Markus

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2958-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2957-4Published: 06 January 2023

  • Softcover ISBN: 978-1-0716-2960-4Published: 07 January 2024

  • eBook ISBN: 978-1-0716-2958-1Published: 05 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 282

  • Number of Illustrations: 9 b/w illustrations, 55 illustrations in colour

  • Topics: Cell Biology

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