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Proteoglycans

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Nikos K. Karamanos

    1. Biochemistry, Biochemical Analysis and Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras, Greece

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2619)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Glycosaminoglycans: Strategies for Detection and Structure Characterization

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Detection of Glycosaminoglycans in Biological Specimens

      • Shaukat A. Khan, F. N. U. Nidhi, Paige C. Amendum, Shunji Tomatsu
      Pages 3-24

    3. Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization of Chondroitin Sulfate Proteoglycans

      • Tadahisa Mikami, Hiroshi Kitagawa
      Pages 25-38

    4. Proteoglycans: A Tool for Detecting Hyaluronan by ELISA-Like Methods

      • Daiana L. Vitale, Rosalía I. Cordo-Russo, Laura Alaniz
      Pages 39-52

    5. Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix

      • Arianna Parnigoni, Paola Moretto, Simona Rovera, Manuela Viola, Evgenia Karousou, Alberto Passi et al.
      Pages 53-60

    6. Urinary Glycosaminoglycans: Characterization and Quantification

      • Naima Fdil, Es-Said Sabir
      Pages 61-69

    7. HPLC-MS Characterization of Tissue-Derived Heparan Sulfate and Chondroitin Sulfate

      • Gábor Tóth, Lilla Turiák
      Pages 71-90

    8. Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues

      • Chrysostomi Gialeli, Isabel Gonçalves
      Pages 91-98

    9. Studying the Effects of Glycosaminoglycans in Cell Morphological Aspect with Scanning Electron Microscopy

      • Christos Koutsakis, Marco Franchi, Anastasia-Gerasimoula Tavianatou, Valentina Masola, Nikos K. Karamanos
      Pages 99-106

  3. Proteoglycans: Separation, Analysis, and Interactions

    1. Front Matter

      Pages 107-107
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    2. Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate Immunity

      • Jinyang Zeng-Brouwers, Lisa Sophie Huber, Rosetta Merline, Jonel Trebicka, Malgorzata Wygrecka, Liliana Schaefer
      Pages 109-124

    3. Analysis of Extracellular Vesicle-Associated Proteoglycans

      • Juliana Poças, Mattias Belting
      Pages 125-139

    4. Analysis of Aggrecan Glycanation by Western Blot in Cell Culture

      • Chiara Gramegna Tota, Alessandra Leone, Antonio Rossi, Chiara Paganini
      Pages 141-151

    5. Repulsive Scaling Replica Exchange Molecular Dynamics in Modeling Protein-Glycosaminoglycan Complexes

      • Mateusz Marcisz, Martyna Maszota-Zieleniak, Sergey A. Samsonov
      Pages 153-167

    6. In Vitro Investigation of Hyaluronan/CD44 Network

      • Theodore Karalis, Paraskevi Heldin, Spyros S. Skandalis
      Pages 169-186

    7. Separation and Identification of Native Proteoglycans by Composite Agarose-Polyacrylamide Gel Electrophoresis and Immunoblotting

      • James Melrose
      Pages 187-209

    8. Building, Visualizing, and Analyzing Glycosaminoglycan–Protein Interaction Networks

      • Sylvie Ricard-Blum
      Pages 211-224

  4. Proteoglycans Related Molecules (Enzymes, Inhibitors, and Epigenetics)

    1. Front Matter

      Pages 225-225
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    2. An Enzymatic Activity Assay for Heparanase That Is Useful for Evaluating Clinically Relevant Inhibitors and Studying Kinetics

      • Edward Hammond, Vito Ferro
      Pages 227-238
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Keywords

About this book

This volume is a comprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs. Chapters are divided into three parts detailing GAGs in biological specimens, protocols for the evaluation of the in vitro and in vivo effects of PGs/GAGs, and protocols for compounds related with the metabolic enzymes, epigenetic regulation, and PGs/GAGs-based inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Cutting-edge and thorough, Proteoglycans: Methods and Protocols aims to provide information on the elucidated the structural and functional aspects of the complex matrix macromolecules such as the proteoglycans and glycosaminoglycans.

 

Editors and Affiliations

  • Biochemistry, Biochemical Analysis and Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras, Greece

    Nikos K. Karamanos

Bibliographic Information

  • Book Title: Proteoglycans

  • Book Subtitle: Methods and Protocols

  • Editors: Nikos K. Karamanos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2946-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2945-1Published: 21 January 2023

  • Softcover ISBN: 978-1-0716-2948-2Published: 21 January 2024

  • eBook ISBN: 978-1-0716-2946-8Published: 20 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 317

  • Number of Illustrations: 8 b/w illustrations, 59 illustrations in colour

  • Topics: Biochemistry, general, Chemistry/Food Science, general

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