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Protein Arginylation

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Anna S. Kashina

    1. Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2620)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Protein Arginylation: Milestones of Discovery

    • Anna S. Kashina
    Pages 1-13

  3. Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase

    • Hideko Kaji, Akira Kaji
    Pages 15-20

  4. Arginyltransferase: A Personal and Historical Perspective

    • Richard L. Soffer
    Pages 21-25

  5. Arginylation in a Partially Purified Fraction of 150 k xg Supernatants of Axoplasm and Injured Vertebrate Nerves

    • Nicholas A. Ingoglia
    Pages 27-34

  6. Preparation of ATE1 Enzyme from Native Mammalian Tissues

    • Anna S. Kashina
    Pages 35-39

  7. Correlated Measurement of Endogenous ATE1 Activity on Native Acceptor Proteins in Tissues and Cultured Cells to Detect Cellular Aging

    • Hideko Kaji, Akira Kaji
    Pages 41-50

  8. Assaying the Posttranslational Arginylation of Proteins in Cultured Cells

    • Mauricio R. Galiano, Marta E. Hallak
    Pages 51-61

  9. Assaying ATE1 Activity in Yeast by β-Gal Degradation

    • Anna S. Kashina
    Pages 63-70

  10. Assaying Arginylation Activity in Cell Lysates Using a Fluorescent Reporter

    • Akhilesh Kumar, Fangliang Zhang
    Pages 71-80

  11. Assaying Intracellular Arginylation Activity Using a Fluorescent Reporter

    • Brittany MacTaggart, Anna S. Kashina
    Pages 81-85

  12. Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays

    • Junling Wang, Anna S. Kashina
    Pages 87-91

  13. Preparation of tRNAArg for Arginylation Assay by In Vitro Transcription

    • Irem Avcilar-Kucukgoze, Anna S. Kashina
    Pages 93-99

  14. Preparation of an Enriched tRNAArg Fraction for Arginylation by Expression in E. coli

    • Irem Avcilar-Kucukgoze, Howard Gamper Jr, Ya-Ming Hou, Anna S. Kashina
    Pages 101-106

  15. Enzymatic Aminoacylation of tRNAArg Using Recombinant Arg-tRNA Synthetase

    • Irem Avcilar-Kucukgoze, Anna S. Kashina
    Pages 107-111

  16. Assaying ATE1 Activity In Vitro

    • Junling Wang, Anna S. Kashina
    Pages 113-117

  17. High-Throughput Arginylation Assay in Microplate Format

    • Sougata Saha, Junling Wang, Anna S. Kashina
    Pages 119-122

  18. Assaying for Arginyltransferase Activity and Specificity by Peptide Arrays

    • Junling Wang, Anna S. Kashina
    Pages 123-127

  19. Assay of Arginyltransferase Activity by a Fluorescent HPLC Method

    • Koichi Takao
    Pages 129-137

  20. Identification of Arginylated Proteins by Mass Spectrometry

    • Anna S. Kashina, John R. Yates III
    Pages 139-152
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Keywords

About this book

This second edition provides updated and new chapters that detail comprehensive overview of all the existing methods on analyzing protein arginylation. Chapters guide readers through the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Cutting-edge and thorough, Protein Arginylation: Methods and Protocols, Second Edition  aims to be of interest to novice researchers involved in posttranslational arginylation research and researchers working on a broad range of posttranslational modifications.







Editors and Affiliations

  • Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, USA

    Anna S. Kashina

Bibliographic Information

  • Book Title: Protein Arginylation

  • Book Subtitle: Methods and Protocols

  • Editors: Anna S. Kashina

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2942-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2941-3Published: 04 April 2023

  • Softcover ISBN: 978-1-0716-2944-4Published: 04 April 2023

  • eBook ISBN: 978-1-0716-2942-0Published: 03 April 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 297

  • Number of Illustrations: 21 b/w illustrations, 35 illustrations in colour

  • Topics: Chemistry/Food Science, general

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