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Inclusion Bodies

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Julian Kopp

    1. Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

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    You can also search for this editor in PubMed   Google Scholar

  2. Oliver Spadiut

    1. Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2617)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Inclusion Bodies: Status Quo and Perspectives

    • Julian Kopp, Oliver Spadiut
    Pages 1-13

  3. Upstream Processing

    1. Front Matter

      Pages 15-15
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    2. Thermoinducible E. coli for Recombinant Protein Production in Inclusion Bodies

      • Norma A. Valdez-Cruz, Mauricio A. Trujillo-Roldán
      Pages 17-30

    3. High-Throughput Expression of Inclusion Bodies on an Automated Platform

      • Annina Kemmer, Linda Cai, M. Nicolas Cruz Bournazou, Peter Neubauer
      Pages 31-47

    4. Design, Production, and Characterization of Catalytically Active Inclusion Bodies

      • Gizem Ölçücü, Karl-Erich Jaeger, Ulrich Krauss
      Pages 49-74

    5. Bidirectional Promoter Libraries Enable the Balanced Co-expression of Two Target Genes in E. coli

      • Julia Jodlbauer, Lukas Rieder, Anton Glieder, Birgit Wiltschi
      Pages 75-86

    6. Inclusion Body Production in Fed-Batch and Continuous Cultivation

      • Stefan Kittler
      Pages 87-102

    7. Avoiding the All-or-None Response in Gene Expression During E. coli Continuous Cultivation Based on the On-Line Monitoring of Cell Phenotypic Switching Dynamics

      • Frank Delvigne, Lucas Henrion, Vincent Vandenbroucke, Juan Andres Martinez
      Pages 103-120

    8. Optimization of Inclusion Body Formation and Purification in Multi-well Plates

      • Sarah Kamel, Julia Schollmeyer, Anke Kurreck, Peter Neubauer
      Pages 121-138

  4. Downstream Processing

    1. Front Matter

      Pages 139-139
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    2. High Pressure Homogenization for Inclusion Body Isolation

      • Julian Ebner, Viktor Sedlmayr, Robert Klausser
      Pages 141-154

    3. Development of Solubilization and Refolding Buffers

      • Mohammad Roufarshbaf, Vajihe Akbari
      Pages 155-164

    4. Unit Operation-Spanning Investigation of the Redox System

      • Julian Ebner, Diana Humer, Viktor Sedlmayr
      Pages 165-176

    5. Using High Pressure and Alkaline pH for Refolding

      • Ligia Morganti, Rosa Maria Chura-Chambi
      Pages 177-187

    6. Intensification of Inclusion Body Processing via Temperature-Based Refolding

      • Ru Shen Wong, Nurul Nadia Mohamad Alias, Eugene Boon Beng Ong, Mervyn W. O. Liew
      Pages 189-200

    7. Refolding of Proteins Expressed as Inclusion Bodies in E. coli

      • Rashmi Sharma, Anupa Anupa, Anurag S. Rathore
      Pages 201-208

    8. Infrared Spectroscopy for Structure Analysis of Protein Inclusion Bodies

      • Andreas Schwaighofer, Bernhard Lendl
      Pages 209-223
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Keywords

About this book

This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IB-based processes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Inclusion Bodies: Methods and Protocols serves as an ideal resource for facilitating diverse aspects of IB processing.

Editors and Affiliations

  • Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Julian Kopp, Oliver Spadiut

Bibliographic Information

  • Book Title: Inclusion Bodies

  • Book Subtitle: Methods and Protocols

  • Editors: Julian Kopp, Oliver Spadiut

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2930-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2929-1Published: 20 January 2023

  • Softcover ISBN: 978-1-0716-2932-1Published: 20 January 2024

  • eBook ISBN: 978-1-0716-2930-7Published: 19 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 272

  • Number of Illustrations: 8 b/w illustrations, 47 illustrations in colour

  • Topics: Biotechnology, Chemistry/Food Science, general, Biomaterials, Pharmaceutical Sciences/Technology

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