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Vectorology for Optogenetics and Chemogenetics

  • Book
  • © 2023

Overview

Editors:
  1. Mark A.G. Eldridge

    1. NIMH, National Institutes of Health, Behesda, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Adriana Galvan

    1. Department of Neurology, School of Medicine, and Emory National Primate Research Center, Emory University, Atlanta, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 195)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Vectors Used in Systems Neuroscience Research

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Production, Testing, and Verification of Lentivirus for Regional Targeting in the Old-World Monkey Brain

      • Walter Lerchner, Alyssa Luz-Ricca, Kiana Dash, Violette DerMinassian, Barry J. Richmond
      Pages 3-15

    3. HiRet/NeuRet Vectors: Lentiviral System for Highly Efficient Gene Transfer Through Retrograde Axonal Transport

      • Kenta Kobayashi, Shigeki Kato, Kazuto Kobayashi
      Pages 17-27

    4. Generation of High-Titer Defective HSV-1 Amplicon Vectors

      • Rachael Neve
      Pages 29-49

    5. Generation and Application of Engineered Rabies Viral Vectors for Neural Circuit Research

      • Masahiro Yamaguchi, Moe Iwata, Riki Kamaguchi, Fumitaka Osakada
      Pages 51-75

    6. Quality Control for Adeno-Associated Viral Vector Production

      • Ina Ersing, Meghan Rego, Chen Wang, Yijun Zhang, Kate Harten DeMaio, Michelle Petrozzi et al.
      Pages 77-101

  3. Vector Modifications and Applications

    1. Front Matter

      Pages 103-103
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    2. Vector Tropism

      • Shih-Heng Chen, Bo He, Sarwyn Singh, Negin P. Martin
      Pages 105-123

    3. Viruses for Systemic Delivery

      • Zikai Wang, Alon Greenbaum, Jennifer B. Treweek
      Pages 125-152

    4. Transcriptomic Definition of Neuron Types

      • Jing He, William R. Stauffer
      Pages 153-167

    5. Enhancers for Selective Targeting

      • Jessica Lin, Jordane Dimidschstein
      Pages 169-184

    6. Pathway-Selective Reversible Perturbations Using a Double-Infection Technique in the Macaque Brain

      • Wim Vanduffel, Tadashi Isa
      Pages 185-205

    7. Pathway-Specific Chemogenetic Manipulation by Applying Ligand to Axonally Expressed DREADDs

      • Maricela X. Martinez, Mitchell R. Farrell, Stephen V. Mahler
      Pages 207-220

  4. Vector Delivery and Verification of Expression

    1. Front Matter

      Pages 221-221
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    2. Convection Enhanced Delivery of Viral Vectors

      • Devon Griggs, Tiphaine Belloir, Jasmine Zhou, Azadeh Yazdan-Shahmorad
      Pages 223-244

    3. Multichannel Microinjector Arrays for Efficient Viral Vector Delivery into Rhesus Monkey Brain

      • Nanami L. Miyazaki, Bing Li, Mark A. G. Eldridge
      Pages 245-261

    4. Methods to Verify Expression and Function of DREADDs Using PET

      • Yuji Nagai, Takafumi Minamimoto
      Pages 263-273

    5. Reporter Selection and Postmortem Methods to Verify Transgene Expression

      • Kate S. Heffernan, Yoland Smith, Adriana Galvan
      Pages 275-292

    6. Considerations for the Use of Viral Vectors in Nonhuman Primates

      • Martin Bohlen, Sébastien Tremblay
      Pages 293-329
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Keywords

About this book

This volume provides an overview of the viral vectors and how they are applied to chemogenetic and optogenetic tools to the study of neural circuits. The chapters in this book are organized into three  parts detailing (1) viral vectors, (2) vector modifications and applications, and (3) practical advice for the delivery of viral vectors, verification of injection accuracy, and monitoring of transgene expression. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

Authoritative and cutting-edge, Vectorology for Optogenetics and Chemogenetics aims to be a useful practical guide to researches to help further their study in this field.


Editors and Affiliations

  • NIMH, National Institutes of Health, Behesda, USA

    Mark A.G. Eldridge

  • Department of Neurology, School of Medicine, and Emory National Primate Research Center, Emory University, Atlanta, USA

    Adriana Galvan

Bibliographic Information

  • Book Title: Vectorology for Optogenetics and Chemogenetics

  • Editors: Mark A.G. Eldridge, Adriana Galvan

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-2918-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2023

  • Hardcover ISBN: 978-1-0716-2917-8Published: 07 February 2023

  • Softcover ISBN: 978-1-0716-2920-8Published: 07 February 2024

  • eBook ISBN: 978-1-0716-2918-5Published: 06 February 2023

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIII, 333

  • Number of Illustrations: 7 b/w illustrations, 64 illustrations in colour

  • Topics: Neurosciences

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