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Glycolipids

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Kazuya Kabayama

    1. Department of Chemistry, Graduate School of Science, Osaka University, Osaka, Japan

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  2. Jin-ichi Inokuchi

    1. Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2613)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
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  2. Immunomodulatory Functions of α-GalCer and a Derivative, α-Carba-GalCer

    • Masashi Satoh, Kazuya Iwabuchi
    Pages 1-11

  3. Chemical Synthesis and Molecular Interaction Analysis of α-Galactosyl Ceramide Derivatives as CD1d Ligands

    • Kazunari Ueki, Kodai Sueyoshi, Shinsuke Inuki, Yukari Fujimoto
    Pages 13-22

  4. Immunomodulatory Functions of Glycolipids from Pathogens

    • Carla Guenther, Miyuki Watanabe, Sho Yamasaki
    Pages 23-31

  5. Chemical Synthesis and Immunomodulatory Functions of Bacterial Lipid As

    • Atsushi Shimoyama, Koichi Fukase
    Pages 33-53

  6. Innovative Vaccine Strategy: Self-Adjuvanting Conjugate Vaccines

    • Yoshiyuki Manabe, Koichi Fukase
    Pages 55-72

  7. Chemical Synthesis of Phosphatidylglucoside

    • Koki Kano, Nozomi Ishii, Peter Greimel, Ichiro Matsuo
    Pages 73-78

  8. Sialidase-Resistant Ganglioside GM3 Analogues: Evaluation of Biological Activity

    • Go Hirai
    Pages 79-87

  9. Chemical Synthesis of Gangliosides

    • Akihiro Imamura, Hiromune Ando, Hideharu Ishida
    Pages 89-100

  10. Sialyltransferase Activity Assay for Ganglioside GM3 Synthase

    • Kei-ichiro Inamori, Takahiro Nitta, Fumi Shishido, Soichiro Watanabe, Isao Ohno, Jin-ichi Inokuchi
    Pages 101-110

  11. Construction of Sphingolipid Remodeled Cells by Genome Editing

    • Toshiyuki Yamaji, Yuta Homma
    Pages 111-125

  12. Mass Spectrometry of Neutral Glycosphingolipids

    • Akemi Suzuki, Atit Silsirivanit, Takashi Watanabe, Junko Matsuda, Kei-ichiro Inamori, Jin-ichi Inokuchi
    Pages 127-144

  13. Structural Analyses of the Glycolipids in Lipid Rafts

    • Yusuke Suzuki, Kazuya Kabayama
    Pages 145-152

  14. Extraction, Purification, and Chemical Degradation of LPS from Gut Microbiota Strains

    • Molly Dorothy Pither, Alba Silipo, Antonio Molinaro, Flaviana Di Lorenzo
    Pages 153-179

  15. NMR Analysis of Mammalian Glycolipids

    • Yoshiki Yamaguchi
    Pages 181-188

  16. Distribution of Glycolipids in the Plasma Membrane Monitored by Specific Probes in Combination with Sodium Dodecyl Sulfate-Digested Freeze-Fracture Replica Labeling (SDS-FRL)

    • Motohide Murate, Toshihide Kobayashi
    Pages 189-202

  17. Biochemical and Microscopic Analyses for Sphingolipids and Its Related Molecules in Phagosomes

    • Hitoshi Nakayama, Kei Hanafusa, Kazuhisa Iwabuchi
    Pages 203-214

  18. Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membranes

    • Kenichi G. N. Suzuki
    Pages 215-227

  19. Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi

    • Makoto Ito, Yohei Ishibashi, Takashi Watanabe, Jun Iwaki, Toyohisa Kurita, Nozomu Okino
    Pages 229-256

  20. Molecular Dynamics of Glycolipids in Liposomes

    • Tomokazu Yasuda, J. Peter Slotte, Michio Murata, Shinya Hanashima
    Pages 257-270
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Keywords

About this book

This detailed volume presents a variety of specialized analytical methods on a wide range of glycolipids. From sphingoglycolipids to lipid A, an immune system stimulant present in lipopolysaccharide, the book delves into how glycolipid structure on the surface of the cell membrane, which characterizes each organism, is crucial for the coexistence or repulsion between organisms. In addition, glycolipids play an important role in infections in animals and other organisms by targeting host cell membranes, if enveloped viruses are considered as living organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Glycolipids: Methods and Protocols serves as an ideal guide to themost advanced glycolipid research in an effort to help researchers make their research more fruitful.

Editors and Affiliations

  • Department of Chemistry, Graduate School of Science, Osaka University, Osaka, Japan

    Kazuya Kabayama

  • Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan

    Jin-ichi Inokuchi

Bibliographic Information

  • Book Title: Glycolipids

  • Book Subtitle: Methods and Protocols

  • Editors: Kazuya Kabayama, Jin-ichi Inokuchi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2910-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2909-3Published: 02 January 2023

  • Softcover ISBN: 978-1-0716-2912-3Published: 29 December 2022

  • eBook ISBN: 978-1-0716-2910-9Published: 01 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 302

  • Topics: Cell Biology, Lipidology

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