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Cell Migration in Three Dimensions

  • Book
  • © 2023

Overview

Editors:
  1. Coert Margadant

    1. Department of Medical Oncology, Cancer Center Amsterdam, Amsterdam University Medical Center, Amsterdam, The Netherlands

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2608)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Cell Migration in Three Dimensions

    • Coert Margadant
    Pages 1-14

  3. The Cell Biology of Cell Migration

    1. Front Matter

      Pages 15-15
      Download chapter PDF

    2. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis

      • Clotilde Huet-Calderwood, Felix Rivera-Molina, Derek Toomre, David A. Calderwood
      Pages 17-38

    3. Probing the ER-Focal Adhesion Link During Cell Migration

      • Noemi A. Guadagno, Cinzia Progida
      Pages 39-50

    4. Mapping the Localization of Proteins Within Filopodia Using FiloMap

      • Guillaume Jacquemet
      Pages 51-61

    5. Extended Methods for 2D Confinement

      • Juan M. García-Arcos, Kevin Gateau, Larisa Venkova, Matthieu Piel
      Pages 63-81

    6. Visualization of Exosome Release and Uptake During Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63

      • Bong H. Sung, Alissa M. Weaver
      Pages 83-96

    7. Approaches to Determine Nuclear Shape in Cells During Migration Through Collagen Matrices

      • Martin Svoren, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, Katarina Wolf
      Pages 97-114Open Access

  4. Developmental Model Systems to Assess Cell Migration During Morphogenesis

    1. Front Matter

      Pages 115-115
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    2. Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila

      • Maik C. Bischoff, Sven Bogdan
      Pages 117-129

    3. Whole-Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells

      • Antonius L. van Boxtel
      Pages 131-145

    4. Live-Imaging Analysis of Epithelial Zippering During Mouse Neural Tube Closure

      • Matteo A. Molè, Gabriel L. Galea, Andrew J. Copp
      Pages 147-162

    5. Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila

      • Sofia J. Araújo, Marta Llimargas
      Pages 163-182

    6. A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland

      • Edouard Hannezo, Colinda L. G. J. Scheele
      Pages 183-205Open Access

    7. Analysis of Integrin-Dependent Melanoblast Migration During Development

      • Amanda Haage, Guy Tanentzapf
      Pages 207-221

  5. Cell Migration in Cancers and the Tumor Microenvironment

    1. Front Matter

      Pages 223-223
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    2. Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices

      • David Remy, Anne-Sophie Macé, Philippe Chavrier, Pedro Monteiro
      Pages 225-246

    3. Analysis of Energy-Driven Leader-Follower Hierarchy During Collective Cancer Cell Invasion

      • Jian Zhang, Cynthia A. Reinhart-King
      Pages 247-262

    4. Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids

      • Konstadinos Moissoglu, Stephen J. Lockett, Stavroula Mili
      Pages 263-280Open Access
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Keywords

About this book

This detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration. The book focuses on the cell biology of cell migration, developmental model systems to assess cell migration during morphogenesis, cell migration in cancers and the tumor micro-environment, as well as blood vessel formation and interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cell Migration in Three Dimensions provides a solid foundation for scientists of different disciplines to investigate cell migration in biological processes. 


Chapters 7, 12, 16, 17, 19, 22, and 24 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Department of Medical Oncology, Cancer Center Amsterdam, Amsterdam University Medical Center, Amsterdam, The Netherlands

    Coert Margadant

Bibliographic Information

  • Book Title: Cell Migration in Three Dimensions

  • Editors: Coert Margadant

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2887-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2886-7Published: 19 January 2023

  • Softcover ISBN: 978-1-0716-2889-8Published: 19 January 2024

  • eBook ISBN: 978-1-0716-2887-4Published: 18 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 472

  • Number of Illustrations: 18 b/w illustrations, 102 illustrations in colour

  • Topics: Cell Biology, Cell Biology

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