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Transposable Elements

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2607)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
  2. Detecting Horizontal Transfer of Transposons

    • James D. Galbraith, Atma M. Ivancevic, Zhipeng Qu, David L. Adelson
    Pages 45-62
  3. A Pangenome Approach to Detect and Genotype TE Insertion Polymorphisms

    • Cristian Groza, Guillaume Bourque, Clément Goubert
    Pages 85-94
  4. Experimental Validation of Transposable Element Insertions Using the Polymerase Chain Reaction (PCR)

    • Miriam Merenciano, Marta Coronado-Zamora, Josefa González
    Pages 95-114
  5. Genome-Wide Young L1 Methylation Profiling by bs-ATLAS-seq

    • Claude Philippe, Gael Cristofari
    Pages 127-150
  6. Targeted Nanopore Resequencing and Methylation Analysis of LINE-1 Retrotransposons

    • Arpita Sarkar, Sophie Lanciano, Gael Cristofari
    Pages 173-198
  7. Affinity-Based Interactome Analysis of Endogenous LINE-1 Macromolecules

    • Luciano H. Di Stefano, Leila J. Saba, Mehrnoosh Oghbaie, Hua Jiang, Wilson McKerrow, Maria Benitez-Guijarro et al.
    Pages 215-256
  8. LINE-1 Retrotransposition Assays in Embryonic Stem Cells

    • Marta Garcia-Cañadas, Francisco J. Sanchez-Luque, Laura Sanchez, Johana Rojas, Jose L. Garcia Perez
    Pages 257-309
  9. Experimental Approaches to Study Somatic Transposition in Drosophila Using Whole-Genome DNA Sequencing

    • Marius van den Beek, Natalia Rubanova, Katarzyna Siudeja
    Pages 311-327
  10. Precise and Scarless Insertion of Transposable Elements by Cas9-Mediated Genome Engineering

    • Vivien Marie Weber, Aurélien J. Doucet, Gael Cristofari
    Pages 329-353
  11. Genetic Knockout of TE Insertions by CRISPR-Cas9

    • David M. Simpson, Edward B. Chuong
    Pages 369-379
  12. Back Matter

    Pages 381-382

About this book

The volume presents a small selection of state-of-the-art approaches for studying transposable elements(TE). Chapters guide readers through HTS-based approaches, bioinformatic tools, methods to studyTE protein complexes, and the functional impact on the host. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Transposable Elements: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 

Editors and Affiliations

  • Faculty of Medicine and Dentistry, Blizard Institute, Queen Mary University of London, London, UK

    Miguel R. Branco

  • School of Biological and Behavioural Sciences, Queen Mary University of London, London, UK

    Alexandre de Mendoza Soler

Bibliographic Information

  • Book Title: Transposable Elements

  • Book Subtitle: Methods and Protocols

  • Editors: Miguel R. Branco, Alexandre de Mendoza Soler

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2883-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2882-9Published: 01 December 2022

  • Softcover ISBN: 978-1-0716-2885-0Published: 01 December 2023

  • eBook ISBN: 978-1-0716-2883-6Published: 30 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 382

  • Number of Illustrations: 5 b/w illustrations, 60 illustrations in colour

  • Topics: Genetics and Genomics, Bioinformatics

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access