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Base Editors

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Sangsu Bae

    1. Seoul National University College of Medicine, Seoul, Korea (Republic of)

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    You can also search for this editor in PubMed   Google Scholar

  2. Beomjong Song

    1. Seoul National University College of Medicine, Seoul, Korea (Republic of)

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2606)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Overview of BEs, Diverse Variants of BEs, and Computational Tools for BEs

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Introduction and Perspectives of DNA Base Editors

      • Beomjong Song, Sangsu Bae
      Pages 3-11

    3. Web-Based Computational Tools for Base Editors

      • Gue-Ho Hwang, Sangsu Bae
      Pages 13-22

    4. Prediction of Base Editing Efficiencies and Outcomes Using DeepABE and DeepCBE

      • Jinman Park, Hui Kwon Kim
      Pages 23-32

    5. Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq

      • Daesik Kim
      Pages 33-40

  3. Application of BEs for Disease Modeling

    1. Front Matter

      Pages 41-41
      Download chapter PDF

    2. Base Editing of Human Hematopoietic Stem Cells

      • Jing Zeng, Gabriele Casirati, My Anh Nguyen, Pietro Genovese, Daniel E. Bauer
      Pages 43-62

    3. A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells

      • Xiaohui Zhang, Yuting Guan, Dali Li
      Pages 63-72

    4. Functional Analysis of Variants in BRCA1 Using CRISPR Base Editors

      • Ji-Eun See, Yongsub Kim
      Pages 73-85

    5. Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice

      • Hiroki Sasaguri
      Pages 87-97

    6. Targeted Mutagenesis in Mice Using a Base Editor

      • Tae Yeong Jeong, Soo-Yeon Lim, Je Kyung Seong, Kyoungmi Kim
      Pages 99-119

  4. Therapeutic Base Editing Strategies

    1. Front Matter

      Pages 121-121
      Download chapter PDF

    2. Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor

      • Seu-Na Lee, Hong-Su Jang, Jae-Sung Woo
      Pages 123-133

    3. Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors

      • Colin K. W. Lim, Angelo J. Miskalis, Pablo Perez-Pinera, Thomas Gaj
      Pages 135-158

    4. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)

      • Dong Yang, Chun-Qing Song
      Pages 159-170

    5. Ex Vivo Base Editing Therapy with Chemically Derived Hepatic Progenitors

      • Yohan Kim, Jaemin Jeong, Dongho Choi
      Pages 171-178

    6. Application of Base Editor-Mediated Genome Editing in Mouse Retina

      • Jun Wu, Chang Sik Cho, Dong Hyun Jo, Jeong Hun Kim
      Pages 179-188

  5. Use of BEs in Plants and Bacteria

    1. Front Matter

      Pages 189-189
      Download chapter PDF

    2. High-Throughput Base Editing-Mediated Artificial Evolution Streamlines Trait Gene Identification in Rice

      • Fang Yan, Man Yu, Meixia Wang, Huanbin Zhou
      Pages 191-202
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Keywords

About this book

This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Base Editors: Methods and Protocols serves as an ideal guide for researchers looking to use base editors to continue their studies in an array of fields.

Editors and Affiliations

  • Seoul National University College of Medicine, Seoul, Korea (Republic of)

    Sangsu Bae, Beomjong Song

Bibliographic Information

  • Book Title: Base Editors

  • Book Subtitle: Methods and Protocols

  • Editors: Sangsu Bae, Beomjong Song

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2879-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2878-2Published: 03 January 2023

  • Softcover ISBN: 978-1-0716-2881-2Published: 04 January 2024

  • eBook ISBN: 978-1-0716-2879-9Published: 02 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 235

  • Number of Illustrations: 12 b/w illustrations, 36 illustrations in colour

  • Topics: Biological Techniques, Genetics and Genomics

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