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The Plant Cytoskeleton

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Patrick J. Hussey

    1. Department of Biosciences, University of Durham, Durham, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Pengwei Wang

    1. College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2604)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Quantification of Microtubule-Bundling Activity of MAPs Using TIRF Microscopy

    • Sharol Schmidt-Marcec, Austin Ross, Andrei Smertenko
    Pages 1-12

  3. Actin: Static and Dynamic Studies

    • Huaqiang Ruan, Sha Zhang, Yi Zhang, Haiyun Ren
    Pages 13-24

  4. 3D Visualization of Microtubules in Epidermal Pavement Cells

    • Amir J. Bidhendi, Bara Altartouri, Anja Geitmann
    Pages 25-42

  5. Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells

    • Sandra Klemm, Jonas Buhl, Birgit Möller, Katharina Bürstenbinder
    Pages 43-61

  6. Single-Cell Confinement Methods to Study Plant Cytoskeleton

    • Pauline Durand-Smet, Antoine Chevallier, Léia Colin, Alice Malivert, Isaty Melogno, Olivier Hamant
    Pages 63-75

  7. Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures

    • Eduard Belausov, Vikas Dwivedi, Sela Yechezkel, Sefi Bar-Sinai, Einat Sadot
    Pages 77-88

  8. Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography

    • Janice Pennington, Marisa S. Otegui
    Pages 89-102

  9. Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana)

    • Yuh-Ru Julie Lee, Calvin H. Huang, Bo Liu
    Pages 103-111

  10. Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis

    • Sabine Müller
    Pages 113-125

  11. Expansion Microscopy of Plant Cells (PlantExM)

    • Timothy J. Hawkins, Joanne L. Robson, Bethany Cole, Simon J. Bush
    Pages 127-142

  12. Microfluidic Device for High-Resolution Cytoskeleton Imaging and Washout Assays in Physcomitrium (Physcomitrella) patens

    • Mari W. Yoshida, Elena Kozgunova
    Pages 143-158

  13. Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens

    • Yuji Hiwatashi, Takashi Murata
    Pages 159-171

  14. Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools

    • Fatima Cvrčková, Radek Bezvoda
    Pages 173-191

  15. Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata

    • Zoe Barr, Jens Tilsner
    Pages 193-202

  16. Studying Nuclear Dynamics in Response to Actin Disruption in Planta

    • Joseph F. McKenna, Katja Graumann
    Pages 203-214

  17. Cytoskeleton Remodeling in Arabidopsis Stigmatic Cells Following Pollination

    • Lucie Riglet, Isabelle Fobis-Loisy
    Pages 215-225

  18. Investigation of ROP GTPase Activity and Cytoskeleton Dynamics During Tip Growth in Root Hairs and Pollen Tubes

    • Lei Zhu, Ying Fu
    Pages 227-235

  19. Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study

    • Teresa Braga, Fernando Vaz Dias, Marta Fratini, Susana Serrazina, Ingo Heilmann, Rui Malhó
    Pages 237-247

  20. Microtubule Reorganization During ABA-Induced Stomatal Closure in Arabidopsis

    • Liru Dou, Xiangfeng Wang, Tonglin Mao
    Pages 249-255
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Keywords

About this book

This detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.

Editors and Affiliations

  • Department of Biosciences, University of Durham, Durham, UK

    Patrick J. Hussey

  • College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China

    Pengwei Wang

Bibliographic Information

  • Book Title: The Plant Cytoskeleton

  • Book Subtitle: Methods and Protocols

  • Editors: Patrick J. Hussey, Pengwei Wang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2867-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2866-9Published: 12 February 2023

  • Softcover ISBN: 978-1-0716-2869-0Published: 12 February 2024

  • eBook ISBN: 978-1-0716-2867-6Published: 11 February 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 369

  • Number of Illustrations: 13 b/w illustrations, 101 illustrations in colour

  • Topics: Plant Sciences, Plant Sciences

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