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SILAC

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2603)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
  2. Dynamic SILAC to Determine Protein Turnover in Neurons and Glia

    • Aline R. Dörrbaum, Erin M. Schuman, Julian D. Langer
    Pages 1-17
  3. SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe

    • Dorothea Anrather, Silvia Bagelova Polakova, Lubos Cipak, Juraj Gregan
    Pages 19-29
  4. pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles

    • So-Fong Cam Ngan, Neil E. McCarthy, Siu Kwan Sze
    Pages 43-58
  5. Quantification of Protein Palmitoylation by Cysteine-SILAC

    • Caiyun Fang, Xiaoqin Zhang, Haojie Lu
    Pages 59-69
  6. A Super-SILAC Approach for Profiling Histone Posttranslational Modifications

    • Roberta Noberini, Elisa Longhi, Tiziana Bonaldi
    Pages 87-102
  7. SILAC-Based Quantitative Phosphoproteomics in Yeast

    • María Luisa Hernáez, Concha Gil
    Pages 103-115
  8. Phosphotyrosine Profiling Using SILAC

    • Keshava K. Datta, Aditi Chatterjee, Harsha Gowda
    Pages 117-125
  9. Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC

    • Alexis Gonneaud, Claude Asselin, Véronique Giroux, François-Michel Boisvert
    Pages 151-161
  10. SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

    • Alistair Langlands, Hamze Beati, H.- Arno J. Müller
    Pages 187-198
  11. Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae

    • Guillermo Aragoneses-Cazorla, Andres Machuca, M. Pilar Buendia-Nacarino, Daniela S. Anunciação, Estefania Garcia-Calvo, Jose L. Luque-Garcia
    Pages 199-207

About this book

This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.

Editors and Affiliations

  • Analytical Chemistry Department, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, Spain

    Jose L. Luque-Garcia

Bibliographic Information

  • Book Title: SILAC

  • Book Subtitle: Methods and Protocols

  • Editors: Jose L. Luque-Garcia

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2863-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2862-1Published: 13 November 2022

  • Softcover ISBN: 978-1-0716-2865-2Published: 14 November 2023

  • eBook ISBN: 978-1-0716-2863-8Published: 12 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 286

  • Number of Illustrations: 10 b/w illustrations, 31 illustrations in colour

  • Topics: Chemistry/Food Science, general, Biological Techniques

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access