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Mechanobiology

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Ronen Zaidel-Bar

    1. Department of Cell and Developmental Biology, Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2600)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  3. Exposing Cells to External Mechanical Forces

    1. Front Matter

      Pages 79-79
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    2. Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber

      • Jason Sedlak, Alisa Clyne
      Pages 81-90

    3. Cell Stretcher Assay to Analyze Mechanoresponses to Cyclic Stretching

      • Robin Püllen, Jens Konrad, Bernd Hoffmann, Rudolf Merkel
      Pages 91-105

    4. Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo

      • Kenji Nishizawa, Claire Chardès, Raphaël Clément, Pierre-François Lenne
      Pages 107-118

  4. Quantitative Characterization of Cell Mechanosensing

    1. Front Matter

      Pages 119-119
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    2. A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity Sensing by Plant’s Roots and Root Hair Cells

      • David Pereira, Thomas Alline, Gaurav Singh, Marie-Edith Chabouté, Atef Asnacios
      Pages 121-131

    3. Photoresponsive Hydrogels for Studying Mechanotransduction of Cells

      • Keun-Young Park, David J. Odde, Mark D. Distefano
      Pages 133-153

    4. Analysing Mechanically Evoked Currents at Cell-Substrate Junctions

      • Surabhi Shrestha, Jessica Richardson, Kate Poole
      Pages 155-167

    5. Quantifying Strain-Sensing Protein Recruitment During Stress Fiber Repair

      • Shailaja Seetharaman, Stefano Sala, Margaret L. Gardel, Patrick W. Oakes
      Pages 169-182

  5. Measuring Forces Applied by Cells on the Extracellular Matrix

    1. Front Matter

      Pages 183-183
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    2. Quantification of Invadopodia Formation and Matrix Degradation Activity

      • Jubina Balan Venghateri, Benjamin Geiger
      Pages 185-196

    3. Measuring Cellular Traction Forces with Micropillar Arrays

      • Malak Amer, Haguy Wolfenson
      Pages 197-206

    4. Imaging Cell Adhesive Force at the Single Molecule Level

      • Ying Tu, Xuefeng Wang
      Pages 207-217

  6. Force Measurement Inside Cells

    1. Front Matter

      Pages 219-219
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Keywords

About this book

This detailed book collects methodologies exploring mechanobiology, the involvement of mechanical forces in cell fate specification and in controlling single and collective cell behaviors such as directed migration, morphogenesis, wound healing, and the immune response. The volume features methods to quantify the mechanical properties of cells and adhesion proteins, to expose cells to external mechanical forces, to quantitatively characterize mechano-responses at various scales, to measure forces applied by cells on the extracellular matrix, as well as chapters on force measurement inside cells, probing cell signaling and gene expression in response to force, and biophysical modeling of cell shape and protein dynamics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Mechanobiology: Methods and Protocols aims to provide meaningful tools for cell and developmental biologists approaching the study of cell and tissue dynamics from a mechanobiological perspective, molecular biologists interested in the effects of force on proteins, as well as for cancer biologists and biophysicists.

Editors and Affiliations

  • Department of Cell and Developmental Biology, Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

    Ronen Zaidel-Bar

Bibliographic Information

  • Book Title: Mechanobiology

  • Book Subtitle: Methods and Protocols

  • Editors: Ronen Zaidel-Bar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2851-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2850-8Published: 02 January 2023

  • Softcover ISBN: 978-1-0716-2853-9Published: 28 December 2022

  • eBook ISBN: 978-1-0716-2851-5Published: 01 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 343

  • Number of Illustrations: 8 b/w illustrations, 96 illustrations in colour

  • Topics: Biomechanics, Biological and Medical Physics, Biophysics, Cell Biology

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