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DNA-Protein Interactions

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2599)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
  2. Enhanced Yeast One-Hybrid Assays to Study Protein–DNA Interactions

    • Anna Berenson, Juan Ignacio Fuxman Bass
    Pages 11-20
  3. Chromatin Immunoprecipitation on Fixed Tissues and Cell Lines

    • Dimitrius Pramio, Deborah Schechtman
    Pages 21-31
  4. ChIP-Seq Protocol for In Vitro Cell Differentiation Systems

    • Jacqueline Copeland, Fjodor Merkuri, Marcos Simoes-Costa
    Pages 49-58
  5. Dissecting Locus-Specific Chromatin Interactions by CRISPR CAPTURE

    • Giovanni A. Botten, Michael Lee Jr, Jian Xu
    Pages 69-97
  6. Rapid Single-Pot Assembly of Modular Chromatin Proteins for Epigenetic Engineering

    • Karmella A. Haynes, J. Harrison Priode
    Pages 191-214
  7. Mapping Transcription Regulation with Run-on and Sequencing Data Using the Web-Based tfTarget Gateway

    • Nating Wang, Zhong Wang, Charles G. Danko, Tinyi Chu
    Pages 215-226
  8. In Embryo Gene Reporter Assays for Evaluation of Cis-Regulatory Regions

    • Carolina Purcell Goes, Tatiane Y. Kanno, C. Y. Irene Yan
    Pages 227-239
  9. Fishing for Developmental Regulatory Regions: Zebrafish Tissue-Specific ATAC-seq

    • Rekha M. Dhillon-Richardson, Alexandra K. Haugan, Megan L. Martik
    Pages 271-282

About this book

This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, DNA-Protein Interactions: Methods and Protocols aims to present genome-wide techniques that will complement the biochemistry-based protocols to aid researchers in their studies. 

Editors and Affiliations

  • Department of Molecular Biology and Genetics, Cornell University, Ithaca, USA

    Marcos Simoes-Costa

Bibliographic Information

  • Book Title: DNA-Protein Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Marcos Simoes-Costa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2847-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2846-1Published: 26 November 2022

  • Softcover ISBN: 978-1-0716-2849-2Published: 26 November 2023

  • eBook ISBN: 978-1-0716-2847-8Published: 25 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 284

  • Number of Illustrations: 10 b/w illustrations, 54 illustrations in colour

  • Topics: Genetics and Genomics, Chemistry/Food Science, general

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access