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Chemokine-Glycosaminoglycan Interactions

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Alexandra R. Lucas

    1. Biodesign Institute, Arizona State University, Tempe, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2597)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy

    • Laura Kutz, Tong Zhou, Qi Chen, Hua Zhu
    Pages 1-9

  3. Remote Ischemic Pre-Conditioning (RIPC)

    • Kyung Eun Lee, Jongsoo Kim, Ki Ho Park, Jianjie Ma, Hua Zhu
    Pages 11-18

  4. Mouse Corneal Transplantation

    • Peng Chen, Ki Ho Park, Liqiang Zhang, Alexandra R. Lucas, Heather L. Chandler, Hua Zhu
    Pages 19-24

  5. Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant

    • Yanlin Ma, Hao Chen
    Pages 25-38

  6. Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine–GAG Interaction and Therapeutic Blockade

    • Isabela R. Zanetti, Liqiang Zhang, Michelle Burgin, Jacquelyn Kilbourne, Jordan R. Yaron, David Fonseca et al.
    Pages 39-58

  7. Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion

    • Sanne L. Maas, Remco T. A. Megens, Emiel P. C. van der Vorst
    Pages 59-75

  8. In Vitro (Trans)Migration Experiment Using Chemokines as Stimulatory Factor

    • Sanne L. Maas, Emiel P. C. van der Vorst
    Pages 77-87

  9. The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins

    • Wesley M. Tierney, Ian B. Hogue
    Pages 89-104

  10. Flow Cytometry Analysis of Immune Cell Responses

    • Kavita R. Manhas, Joseph N. Blattman
    Pages 105-120

  11. Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence

    • Bruno Hernaez, Antonio Alcamí
    Pages 121-129

  12. Protein Microarrays and their Fabrication

    • Mahasish Shome, Joshua Labaer
    Pages 131-142

  13. NMR Methods for Characterization of Glycosaminoglycan–Chemokine Interactions

    • Vitor H. Pomin, Krishna Rajarathnam
    Pages 143-157

  14. Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG–Protein Interactions

    • Amrita Basu, Ryan J. Weiss
    Pages 159-176

  15. Isolation and Compositional Analysis of Glycosaminoglycans

    • Stephanie Archer-Hartmann, Lauren E. Pepi, Christian Heiss, Parastoo Azadi
    Pages 177-186

  16. How to Design Peptides

    • Joseph Dodd-o, Amanda M. Acevedo-Jake, Abdul-Rahman Azizogli, Vikram Khipple Mulligan, Vivek A. Kumar
    Pages 187-216

  17. Methods to Assess Chemokine Binding and Anti-chemotactic Activity of Virus Proteins

    • Elham Torbati, Gabriella Stuart, Kurt Krause, Chris Brown, Lyn Wise
    Pages 217-234

  18. Producing Biologics with Defined N-Glycosylation in Plants

    • Adrian Esqueda, Qiang Chen
    Pages 235-250

  19. Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery

    • Ryan Boyd, Liqiang Zhang, Petra Fromme
    Pages 251-259

  20. Back Matter

    Pages 261-262
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Keywords

About this book

This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Chemokine-Glycosaminoglycan Interactions: Methods and Protocols serves as an ideal guide for researchers seeking to analyze chemokine and GAG functions, interactions, and molecular mechanisms in vivo and in vitro.

Editors and Affiliations

  • Biodesign Institute, Arizona State University, Tempe, USA

    Alexandra R. Lucas

Bibliographic Information

  • Book Title: Chemokine-Glycosaminoglycan Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Alexandra R. Lucas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2835-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2834-8Published: 15 November 2022

  • Softcover ISBN: 978-1-0716-2837-9Published: 15 November 2023

  • eBook ISBN: 978-1-0716-2835-5Published: 14 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 262

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Immunology, Cell Biology

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