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Difference Gel Electrophoresis

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Kay Ohlendieck

    1. Maynooth University, MAYNOOTH, Ireland

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2596)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xii
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Two-Dimensional Gel Electrophoresis and 2D-DIGE

      • Paula Meleady
      Pages 3-15

  3. Difference Gel Electrophoresis

    1. Front Matter

      Pages 17-17
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    2. Top-Down Proteomics and Comparative 2D-DIGE Analysis

      • Kay Ohlendieck
      Pages 19-38

    3. DIGE Analysis Software and Protein Identification Approaches

      • Paul Dowling
      Pages 39-50

  4. DIGE Applications

    1. Front Matter

      Pages 51-51
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    2. Native DIGE for Quantitative and Functional Analysis of Protein Interactomes

      • Diksha Dani, Norbert A. Dencher
      Pages 53-69

    3. Comparative Two-Dimensional Fluorescence Gel Electrophoresis

      • Doreen Ackermann, Simone König
      Pages 71-81

    4. Identification of Ubiquitination-Associated Proteins Using 2D-DIGE

      • Paul Dowling, Despina Bazou
      Pages 83-96

    5. DIGE-Based Phosphoproteomic Analysis

      • Taras Stasyk, Lukas Alfons Huber
      Pages 97-104

    6. DIGE-Based Biomarker Discovery in Blood Cancers

      • Katie Dunphy, Paul Dowling
      Pages 105-112

    7. DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue

      • Paul Dowling
      Pages 113-118

    8. DIGE Analysis of ProteoMiner™ Fractionated Serum/Plasma Samples

      • Sandra Murphy, Paul Dowling
      Pages 119-125

    9. Comparative 3-Sample 2D-DIGE Analysis of Skeletal Muscles

      • Kay Ohlendieck
      Pages 127-146

    10. Proteomic Identification of Saliva Proteins as Noninvasive Diagnostic Biomarkers

      • Eleanor M. O’Sullivan, Paul Dowling, Dieter Swandulla, Kay Ohlendieck
      Pages 147-167

    11. Analysis of the Saliva Proteome Using 2D-DIGE

      • Paul Dowling, Eleanor M. O’Sullivan
      Pages 169-174

  5. Comparative DIGE Tissue Proteomics

    1. Front Matter

      Pages 175-175
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    2. DIGE Analysis of Clinical Specimens

      • Cecilia Gelfi, Daniele Capitanio
      Pages 177-199

    3. DIGE Analysis of Animal Tissues

      • Alessio Di Luca, Ruth Hamill, Anne Maria Mullen, Giuliano Elia
      Pages 201-216
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Keywords

About this book

The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

 

Editors and Affiliations

  • Maynooth University, MAYNOOTH, Ireland

    Kay Ohlendieck

Bibliographic Information

  • Book Title: Difference Gel Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Kay Ohlendieck

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2831-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2830-0Published: 16 November 2022

  • Softcover ISBN: 978-1-0716-2833-1Published: 16 November 2023

  • eBook ISBN: 978-1-0716-2831-7Published: 15 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 485

  • Number of Illustrations: 8 b/w illustrations, 74 illustrations in colour

  • Topics: Chemistry/Food Science, general

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