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Endophytic Microbes: Isolation, Identification, and Bioactive Potentials

  • Book
  • © 2023

Overview

Editors:
  1. A. Sankaranarayanan

    1. Department of Life Sciences, Sri Sathya Sai University for Human ExcellenceNavanihal, Kamalapura, Kalaburagi, India

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  2. Natarajan Amaresan

    1. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, India

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    You can also search for this editor in PubMed   Google Scholar

  3. Mitesh Kumar Dwivedi

    1. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, India

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Cultivation Methods of Bacterial Endophytes

    1. Front Matter

      Pages 1-1
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    2. Isolation of Endophytic Bacteria from Leaves, Stems, or Roots

      • Joyce Prisca Bakuwa Njoloma
      Pages 3-6

    3. Heat-Treatment Method for Isolation of Bacillus spp. from Plant Tissues

      • T. R. Kavitha, C. Suneetha, T. R. Sunitha
      Pages 7-13

    4. Isolation and Detection of Endophytic Streptomyces from Actinorhizal Casuarina Root Nodules

      • Thirumagal Thirugnanam, Dhanasekaran Dharumadurai
      Pages 15-25

    5. Isolation of Endophytic Fungi from Stems, Leaves, or Roots

      • V. Ambikapathy, N. Sengottaian, A. S. Shijila Rani, S. Babu, A. Anbukumaran
      Pages 27-29

  3. Biochemical Methods for Identification of Endophytes

    1. Front Matter

      Pages 31-31
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    2. Detection of Endophytes by Cultivation Method

      • Paula Rodríguez, David Gonzalez, Sonia Rodríguez Giordano
      Pages 33-42

    3. Detection of Endophytes by Visualization Methods

      • Vijayalakshmi Selvakumar, Karnan Muthusamy, Thenmozhi Manivel, Preethi Gopalsamy, Panneerselvam Annamalai, Ramachandran Chelliah et al.
      Pages 43-49

    4. Detection of Endophytes by Immunological Methods

      • Bankapura Mariyappa Ravikumara, Lakshmaiah Manjunatha, Kittan Subathra, Gummeral Narasareddy, Gangadharaiah Jyothi, Chikkabuddi Prashantha
      Pages 51-69

    5. Detection of Endophytes by Electron Microscope

      • Pradeep Kumar, Simran Rani, Sarita, Amita Suneja Dang, Pooja Suneja
      Pages 71-76

    6. Detection of Endophytes by Reactive Oxygen Staining

      • Simran Rani, Pradeep Kumar, Deepika, Amita Suneja Dang, Pooja Suneja
      Pages 77-81

  4. Molecular Methods for Identification of Endophytes

    1. Front Matter

      Pages 83-83
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    2. Identification of Bacterial Endophytes by 16S rRNA

      • V. Ambikapathy, S. Babu, R. Shanmugapriya, A. Prakash, A. S. Shijila Rani
      Pages 85-87

    3. Identification of Fungal Endophytes by ITS rDNA Technique

      • Pooja Suneja, Pradeep Kumar, Simran Rani, Simran, Amita Suneja Dang
      Pages 89-95

    4. Identification of Fungal Endophytes by 28SrDNA Technique

      • Karnan Muthusamy, Tamilkani Pichai, Vijayalakshmi Selvakumar, Anbukumaran Ananthan, Reshma Devi Ramesh, Leelavathi Lakshmanan et al.
      Pages 97-108

    5. Detection of Endophytes by Molecular Fingerprinting Techniques

      • Lakshmaiah Manjunatha, Gummeral Narasareddy, Bankapura Mariyappa Ravikumar, Leela Saisree Uppala, Rishikesh Kumar, Nanjundappa Manjunatha et al.
      Pages 109-133

    6. Metagenomics and Pyrosequencing Approach

      • Jean Carlos Vencioneck Dutra, Maria do Carmo Pimentel Batitucci
      Pages 135-142

  5. Methods to Proof Endophytism

    1. Front Matter

      Pages 143-143
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    2. Cultivation-Based Studies of Endophytic Fungi in Plants

      • Lizzy A. Mwamburi
      Pages 145-151
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Keywords

About this book

This volume provides basic insight and protocols relating to endophytic microbes. Chapter are divided into five major sections detailing basic isolation, bioactive metabolites production. endophytism, isolation and identification of endophytes, bioactive potentials, and screening of metabolites.

Authoritative and cutting-edge, Endophytic Microbes: Isolation, Identification, and Bioactive Potentials aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

Editors and Affiliations

  • Department of Life Sciences, Sri Sathya Sai University for Human ExcellenceNavanihal, Kamalapura, Kalaburagi, India

    A. Sankaranarayanan

  • C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, India

    Natarajan Amaresan, Mitesh Kumar Dwivedi

Bibliographic Information

  • Book Title: Endophytic Microbes: Isolation, Identification, and Bioactive Potentials

  • Editors: A. Sankaranarayanan, Natarajan Amaresan, Mitesh Kumar Dwivedi

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-0716-2827-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2826-3Published: 26 November 2022

  • Softcover ISBN: 978-1-0716-2829-4Published: 26 November 2023

  • eBook ISBN: 978-1-0716-2827-0Published: 25 November 2022

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XIV, 272

  • Number of Illustrations: 8 b/w illustrations, 24 illustrations in colour

  • Topics: Microbiology

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