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Signal Transduction Immunohistochemistry

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Alexander E. Kalyuzhny

    1. Bio-Techne, Minneapolis, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2593)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Top Five Considerations When Choosing an Antibody

    • Amy Archuleta, Michael D. Browning
    Pages 1-20

  3. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting

    • Biji T. Kurien, R. Hal Scofield
    Pages 21-33

  4. Recent Advances in Chromogens for Immunohistochemistry

    • Bipin Gupta, George Yang, Olga Petrauskene, Marc Key
    Pages 35-50

  5. Overview of Imaging Techniques in Immunohistochemistry

    • Gerald Sedgewick
    Pages 51-82

  6. Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells

    • Pravin J. Mishra, Debabrata Banerjee
    Pages 83-92

  7. Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments

    • Hemant K. Mishra
    Pages 93-112

  8. Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex Immunostaining

    • Jingwen Niu, Jodi Hagen, Feifan Yu, Alexander E. Kalyuzhny, Andrew Tsourkas
    Pages 113-126

  9. Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species

    • Matthew Levin, Zohreh AkhavanAghdam, David Schwartz
    Pages 127-142

  10. 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes

    • Sharla L. White, Amy T. Lam, Hannah D. Buck
    Pages 143-161

  11. Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research

    • Simeon C. Daeschler, Gregory H. Borschel
    Pages 163-169

  12. Protocol and Software for Automated Detection of Lysosome Active “Runs” and “Flights” with Wavelet Transform Approach

    • Kristiana Kandere-Grzybowska, Konstantin Polev, Diana V. Kolygina, Bartosz A. Grzybowski
    Pages 171-195

  13. Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing

    • Christine Goetz, Christopher Hammerbeck, Kristina Boss, Ryan Peterson, Jody Bonnevier
    Pages 197-212

  14. In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-detection of Phosphorylated EGFR in A431 Cultured Cells

    • Jodi Hagen, Ashley Oliver, Alexander E. Kalyuzhny
    Pages 213-220

  15. In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue

    • Ashley Oliver, Jodi Hagen, Sue Yang, Alexander E. Kalyuzhny
    Pages 221-232

  16. Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry

    • Wenfei Kang, Anthony Santella, Eric Rosiek, Maria Pulina, Eric Chan, Ning Fan et al.
    Pages 233-244

  17. Transcription Factor-Mediated Differentiation of Motor Neurons from Human Pluripotent Stem Cells

    • Junjiao Wu, Yu Tang
    Pages 245-258

  18. Immunohistochemical Techniques for Phosphoproteins

    • Berenice L. Sanz Ressel, Alfredo A. Molinolo
    Pages 259-264

  19. Visualizing Cofilin-Actin Filaments by Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in Cells

    • Laurie S. Minamide, Ryan Hylton, Matthew Swulius, James R. Bamburg
    Pages 265-281

  20. Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Formalin-Fixed Paraffin-Embedded Human Brain Sections

    • Clara Muñoz-Castro, Ayush Noori, Bradley T. Hyman, Alberto Serrano-Pozo
    Pages 283-305
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Keywords

About this book

This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination of IHC with In Situ Hybridization (ISH), wavelet transform approach for organelle tracking, transcription factors in human stem cells, differentiation of mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the human brain, tissue fixation, permeabilization, and cryo-preservation, as well as other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.

Editors and Affiliations

  • Bio-Techne, Minneapolis, USA

    Alexander E. Kalyuzhny

Bibliographic Information

  • Book Title: Signal Transduction Immunohistochemistry

  • Book Subtitle: Methods and Protocols

  • Editors: Alexander E. Kalyuzhny

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2811-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2810-2Published: 14 December 2022

  • Softcover ISBN: 978-1-0716-2813-3Published: 14 December 2023

  • eBook ISBN: 978-1-0716-2811-9Published: 13 December 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 328

  • Number of Illustrations: 17 b/w illustrations, 67 illustrations in colour

  • Topics: Biological Techniques, Chemistry/Food Science, general

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