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Type-1 Diabetes

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Anna Moore

    1. Precision Health Program, Michigan State University, East Lansing, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Ping Wang

    1. Precision Health Program, Michigan State University, East Lansing, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2592)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. 3D Optical Molecular Imaging of the Rodent Pancreas by OPT and LSFM

    • Max Hahn, Ulf Ahlgren
    Pages 1-19

  3. Uncommon Transplantation Sites: Transplantation of Islets and Islet Organoids in the Anterior Chamber of the Eye of Rodents and Monkeys

    • Tilo Moede, Sai Bo Bo Tun, Ingo B. Leibiger, Per-Olof Berggren
    Pages 21-36

  4. Generation of Insulin-Producing Multicellular Organoids

    • Laura Mar Fonseca, Fanny Lebreton, Charles-Henri Wassmer, Ekaterine Berishvili
    Pages 37-60

  5. Noninvasive Quantitative PET Imaging in Humans of the Pancreatic Beta-Cell Mass Biomarkers VMAT2 and Dopamine D2/D3 Receptors In Vivo

    • Jason Bini, Richard E. Carson, Gary W. Cline
    Pages 61-74

  6. In Vivo Imaging of Naked and Microencapsulated Islet Cell Transplantation

    • Dian R. Arifin, Jeff W. M. Bulte
    Pages 75-88

  7. Diabetes Retinopathy: New Ways to Detect and Treat

    • Todd A. Lydic, Julia V. Busik
    Pages 89-100

  8. MRI Methods for Imaging Beta-Cell Function in the Rodent Pancreas

    • Pooyan Khalighinejad, Eul Hyun Suh, A. Dean Sherry
    Pages 101-111

  9. MicroRNAs in Type 2 Diabetes: Focus on MicroRNA Profiling in Islets of Langerhans

    • Elaine Cowan, Alexandros Karagiannopoulos, Lena Eliasson
    Pages 113-142

  10. Protocol for Clinical GLP-1 Receptor PET/CT Imaging with [68Ga]Ga-NODAGA-Exendin-4

    • S. Tokgöz, M. Boss, S. Prasad, P. Shah, P. Laverman, M. van Riel et al.
    Pages 143-153

  11. Hyperinsulinemic–Euglycemic Clamp in Conscious Rats Based on the Tail Artery and Vein Catheterization

    • Xiaohuan Liu, LuLu Chen, Xiang Hu
    Pages 155-161

  12. Synthesis of siRNA-Conjugated Dextran-Coated Iron Oxide Nanoparticles for Islet Protection During Transplantation and Noninvasive Imaging

    • Neil Robertson, Ping Wang, Nazanin Talebloo, Kazuhiko Yamada, Anna Moore
    Pages 163-174

  13. Micelle-Based Nanocarriers for Targeted Delivery of Cargo to Pancreas

    • Swati Mishra, Philip R. Streeter
    Pages 175-184

  14. Magnetic Particle Imaging of Transplanted Human Islets Using a Machine Learning Algorithm

    • Aixia Sun, Hasaan Hayat, Simon W. Sanchez, Anna Moore, Ping Wang
    Pages 185-194

  15. In Vivo Bioluminescence for the Detection of the Fate of Pancreatic Islet Organoids Post-transplantation

    • Aixia Sun, Elizabeth Kenyon, Mithil Gudi, Wen Li, Aitor Aguirre, Ping Wang
    Pages 195-206

  16. miRNA Theranostic Nanoparticles Promote Pancreatic Beta Cell Proliferation in Type 1 Diabetes Model

    • Saumya Nigam, Anna Moore, Ping Wang
    Pages 207-218

  17. Isolation and Purification of Human Pancreatic Islets

    • Lingling Wei
    Pages 219-232

  18. Vascularized Islet Transplantation as Composite Islet-Kidney Grafts with Nanoparticle-Labeled Islets in Large Animal Preclinical Transplant Models

    • Kazuhiko Yamada, Daniel L. Eisenson, Xiaojuan Chen, Lei Ji, Michelle R. Santillan, Anna Moore
    Pages 233-249

  19. Back Matter

    Pages 251-252
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Keywords

About this book

This volumes details methods focusing on technological innovation and recent advances in diabetes management. Chapters will guide readers through recent advances, beta-cell regeneration, non-invasive imaging of endogenous, transplanted islets theranostics, microRNA profiling of beta-cells and artificial intelligence, and deep learning algorithms in diabetes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Type-1 Diabetes: Methods and Protocols is for a broad audience including basic researches, clinicians, and physician scientists whose major focus is in diabetes.



Editors and Affiliations

  • Precision Health Program, Michigan State University, East Lansing, USA

    Anna Moore, Ping Wang

Bibliographic Information

  • Book Title: Type-1 Diabetes

  • Book Subtitle: Methods and Protocols

  • Editors: Anna Moore, Ping Wang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2807-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2806-5Published: 13 December 2022

  • Softcover ISBN: 978-1-0716-2809-6Published: 13 December 2023

  • eBook ISBN: 978-1-0716-2807-2Published: 12 December 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 252

  • Number of Illustrations: 5 b/w illustrations, 49 illustrations in colour

  • Topics: Biomedicine, general, Endocrinology

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