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HDAC/HAT Function Assessment and Inhibitor Development

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Oliver H. Krämer

    1. Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2589)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xviii
    Download chapter PDF

  2. In Vivo and 3R Models to Assess HDAC Functions and Effects of HDACi

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Analyzing Lymphoma Development and Progression Using HDACi in Mouse Models

      • Eva-Maria Piskor, René Winkler, Christian Kosan
      Pages 3-15

    3. Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells

      • Miriam Pons, Mandy Beyer
      Pages 17-25

    4. Defined Human Leukemic CD34+ Liquid Cultures to Study HDAC/Transcriptional Repressor Complexes

      • Roland Windisch, Sophie Kreissig, Christian Wichmann
      Pages 27-49

    5. Development of a Cellular Model Mimicking Specific HDAC Inhibitors

      • Lena Hess, Verena Moos, Christian Seiser
      Pages 51-73

    6. Evaluation of Antitumor and On-Target Activity of HDAC Inhibitors with the Zebrafish Embryo Xenograft Model

      • Charlotte Gatzweiler, Johannes Ridinger, Simay Ayhan, Sara Najafi, Heike Peterziel, Olaf Witt et al.
      Pages 75-85

    7. Human Platelet Lysate as Valid Cell Growth Additive to Assess Protein Acetylation

      • Miriam Pons, Roland Conradi, Walburgis Brenner, Oliver H. Krämer
      Pages 87-94

    8. Investigating Physiopathological Roles for Sirtuins in a Mouse Model

      • Shimin Sun, Xiaojiao Xia, Ming Wang, Baohua Liu
      Pages 95-110

    9. Patient-Derived Organoids for In Vivo Validation of In Vitro Data

      • Said Kdimati, Florian Bürtin, Michael Linnebacher, Christina Susanne Mullins
      Pages 111-126

  3. Novel HDAC Inhibitors and Improved Formulations for HDAC Inhibitors

    1. Front Matter

      Pages 127-127
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    2. Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges

      • Marie Kühne, Susanne Hofmann, Henry Lindemann, Zoltán Cseresnyés, Andreas Dzierza, Daniel Schröder et al.
      Pages 129-144

    3. Development of Pyrazine-Anilinobenzamides as Histone Deacetylase HDAC1–3 Selective Inhibitors and Biological Testing Against Pancreas Cancer Cell Lines

      • Mohamed Abdelsalam, Hany S. Ibrahim, Lukas Krauss, Matthes Zessin, Anita Vecchio, Sieglinde Hastreiter et al.
      Pages 145-155

    4. Evaluation of Small-Molecule HDAC Inhibitors Through In Vitro and In Cellulo Approaches

      • Nabanita Nawar, Harsimran Kaur Garcha, Abootaleb Sedighi, Elvin D. de Araujo, Patrick T. Gunning
      Pages 157-177

    5. Synthesis, Biochemical, and Cellular Evaluation of HDAC6 Targeting Proteolysis Targeting Chimeras

      • Salma Darwish, Tino Heimburg, Johannes Ridinger, Daniel Herp, Matthias Schmidt, Christophe Romier et al.
      Pages 179-193

    6. HDACi Delivery Systems Based on Cellulose Valproate Nanoparticles

      • Henry Lindemann, Marie Kühne, Andreas Koschella, Maren Godmann, Thorsten Heinzel, Thomas Heinze
      Pages 195-205

    7. Synthesis and Characterization of Reversible Covalent HDAC4 Inhibitors

      • Anton Frühauf, Benjamin Wolff, Markus Schweipert, Franz-Josef Meyer-Almes
      Pages 207-221

  4. Innovative Techniques to Determine the Impact of Acetylation on Chromatin, DNA Damage, and Cell Stress Responses

    1. Front Matter

      Pages 223-223
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    2. Analysis of Cell Cycle and DNA Compaction Dependent Subnuclear Distribution of Histone Marks

      • Sunil Kumar Pradhan, M. Cristina Cardoso
      Pages 225-239

    3. Analyzing the Effects of HDAC Inhibitors on DNA Damage and Associated Cytotoxicity in Primary Hepatocytes

      • Max J. Carlsson, Jörg Fahrer
      Pages 241-252
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Keywords

About this book

This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors explore the generation and evaluation of novel histone deacetylase inhibitors and new and improved techniques to assess acetylation-dependent molecular mechanisms in vitro and in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to further elucidate this vital area of study.

Editors and Affiliations

  • Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

    Oliver H. Krämer

Bibliographic Information

  • Book Title: HDAC/HAT Function Assessment and Inhibitor Development

  • Book Subtitle: Methods and Protocols

  • Editors: Oliver H. Krämer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2788-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2787-7Published: 19 October 2022

  • Softcover ISBN: 978-1-0716-2790-7Published: 19 October 2023

  • eBook ISBN: 978-1-0716-2788-4Published: 18 October 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVII, 511

  • Number of Illustrations: 39 b/w illustrations, 98 illustrations in colour

  • Topics: Protein Science, Enzymology

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