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Plant Proteostasis

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Provides step-by-step detail essential for reproducible results

Part of the book series: Methods in Molecular Biology (MIMB, volume 2581)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xvi
  2. Ubiquitin Conjugation and Deconjugation Analysis

    1. Front Matter

      Pages 1-1
    2. An In vitro Assay to Recapitulate Hormone-Triggered and SCF-Mediated Protein Ubiquitylation

      • Michael Niemeyer, Jhonny Oscar Figueroa Parra, Luz Irina A. Calderón Villalobos
      Pages 43-56
    3. Analysis of Proteasome-Associated Ubiquitin Ligase Activity

      • Zhishuo Wang, Beatriz Orosa-Puente, Steven H. Spoel
      Pages 57-67
    4. Measuring the DUB Activity of Arabidopsis Deubiquitylating Enzymes

      • Karin Vogel, Marie-Kristin Nagel, Erika Isono
      Pages 69-79
  3. Sumo Conjugation and Deconjugation

    1. Front Matter

      Pages 81-81
    2. SUMO Conjugation and SUMO Chain Formation by Plant Enzymes

      • Konstantin Tomanov, Jose Julian, Ionida Ziba, Andreas Bachmair
      Pages 83-92
    3. Kinetic Analysis of Plant SUMO Conjugation Machinery

      • Laura Castaño-Miquel, L. Maria Lois
      Pages 93-108
    4. Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases

      • Prakash Kumar Bhagat, Dipan Roy, Ari Sadanandom
      Pages 109-119
  4. Analysis of Autophagy

    1. Front Matter

      Pages 121-121
    2. Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii

      • José L. Crespo, María Esther Pérez-Pérez
      Pages 123-134
    3. Detection of Autophagy in Plants by Fluorescence Microscopy

      • Yunting Pu, Diane C. Bassham
      Pages 135-147
    4. Characterization of ATG8-Family Interactors by Isothermal Titration Calorimetry

      • Lorenzo Picchianti, Arthur Sedivy, Yasin Dagdas
      Pages 149-176
  5. Protein Turnover and Stability

    1. Front Matter

      Pages 177-177
    2. Protocols for Studying Protein Stability in an Arabidopsis Protoplast Transient Expression System

      • Séverine Planchais, Laurent Camborde, Isabelle Jupin
      Pages 179-199
    3. Relative Protein Lifetime Measurement in Plants Using Tandem Fluorescent Protein Timers

      • Hongtao Zhang, Eric Linster, Markus Wirtz, Frederica L. Theodoulou
      Pages 201-220
    4. Detection and Quantification of Protein Aggregates in Plants

      • Ujjal Jyoti Phukan, Simon Stael, Amanda Gonçalves, Frank Van Breusegem, Núria S. Coll
      Pages 221-228

About this book

 This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field. 


Editors and Affiliations

  • Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Barcelona, Spain

    L. Maria Lois

  • Faculty of Biology, University of Freiburg, Freiburg, Germany

    Marco Trujillo

Bibliographic Information

  • Book Title: Plant Proteostasis

  • Book Subtitle: Methods and Protocols

  • Editors: L. Maria Lois, Marco Trujillo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2784-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2783-9Published: 23 November 2022

  • Softcover ISBN: 978-1-0716-2786-0Published: 23 November 2023

  • eBook ISBN: 978-1-0716-2784-6Published: 22 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 415

  • Number of Illustrations: 23 b/w illustrations, 57 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access